Department of Biomedical Engineering, Department of Chemistry, Northwestern University, Evanston, IL, 60208, USA.
Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL, 60611, USA.
Small. 2016 Jul;12(28):3810. doi: 10.1002/smll.201670138.
On page 3811, M. Mrksich and co-workers culture cells using self-assembled monolayers presenting cell adhesion ligands and enzyme substrates. A lysis buffer disrupts the cell membranes, releasing enzymes that modify the immobilized substrates. These modifications can be measured with SAMDI mass spectrometry, giving a high-throughput, cell-based assay.
第 3811 页，M. Mrksich 及其同事使用展示细胞黏附配体和酶底物的自组装单层来培养细胞。裂解缓冲液破坏细胞膜，释放出修饰固定化底物的酶。这些修饰可以用 SAMDI 质谱进行测量，从而提供高通量的基于细胞的测定。
