Rincón Camacho Laura, Morandini Leonel, Birba Agustina, Cavallino Luciano, Alonso Felipe, LoNostro Fabiana L, Pandolfi Matias
Laboratorio De Neuroendocrinología Y Comportamiento, Departamento de Biodiversidad y Biología Experimental (DBBE), FCEN, UBA E IBBEA, CONICET-UBA, Ciudad Universitaria, Intendente Güirlades 2160, Ciudad Autónoma De Buenos Aires, C1428EHA, Argentina.
División Ictiología, CONICET, Museo Argentino De Ciencias Naturales, Buenos Aires, C1405DJR, Argentina.
J Morphol. 2016 Oct;277(10):1355-67. doi: 10.1002/jmor.20581. Epub 2016 Jul 20.
Cardinal neon Paracheirodon axelrodi and bloodfin tetra Aphyocharax anisitsi are two species of characids with high trade value as ornamental fish in South America. Although both species inhabit middle water layers, cardinal neon exhibits a tropical distribution and bloodfin tetra a subtropical one. In this work, we carried out an anatomical, histological and immunohistochemical study of the pineal complex of P. axelrodi and A. anisitsi. In both species, the pineal complex consisted of three components, the pineal and parapineal organs and the dorsal sac (DS). The pineal organ was composed of a short, thin pineal stalk (PS), vertically disposed with respect to the upper surface of the telencephalon, and a pineal vesicle (PV), located at the distal end of the PS and attached to the skull by connective tissue. The pineal window (PW), a site in the skull where the luminal information accesses the pineal organ, appeared just above the latter structures. In the epidermis of P. axelrodi's PW, club cells were identified, but were not observed in the epidermis of A. anisitsi's one. With respect to the DS, it appeared to be folded on itself, and was bigger and more folded in A. anisitsi than in P. axelrodi. Immunohistochemical assays revealed the presence of cone opsin-like and rod opsin-like photoreceptor cells in the PS and PV. These results provide a first insight into the morphological assembly of the pineal complex of both species, and contribute to a better understanding of the integration and transduction of light stimuli in characids. J. Morphol. 277:1355-1367, 2016. © 2016 Wiley Periodicals, Inc.
宝莲灯鱼（Paracheirodon axelrodi）和血鳍脂鲤（Aphyocharax anisitsi）是南美洲具有很高商业价值的两种脂鲤科观赏鱼。尽管这两个物种都栖息于中水层，但宝莲灯鱼分布在热带地区，而血鳍脂鲤分布在亚热带地区。在这项研究中我们对宝莲灯鱼和血鳍脂鲤的松果复合体进行了解剖学、组织学和免疫组织化学研究。在这两个物种中，松果复合体均由三个部分组成，即松果体、副松果体和背囊（DS）。松果体由一根短而细的松果体柄（PS）组成，其相对于端脑上表面垂直排列，以及一个松果泡（PV），位于PS的远端并通过结缔组织附着于头骨。松果窗（PW）是头骨上一个腔室信息进入松果体的部位，正好出现在后述结构的上方。在宝莲灯鱼PW的表皮中发现了棒状细胞，但在血鳍脂鲤的PW表皮中未观察到。关于背囊，它似乎自身折叠，血鳍脂鲤中的比宝莲灯鱼中的更大且折叠更多。免疫组织化学分析显示在PS和PV中存在视锥蛋白样和视杆蛋白样光感受器细胞。这些结果首次揭示了这两个物种松果复合体的形态结构，并有助于更好地理解脂鲤科鱼类中光刺激的整合与传导。《形态学杂志》277:1355 - 1367，2016年。© 2016威利期刊公司。
