Zou Nan, Chen Ronghua, Qin Yuhong, Song Shuangyu, Tang Xinglin, Pan Canping
Department of Applied Chemistry, College of Science, China Agricultural University, Beijing, People's Republic of China.
Institute of Pesticide and Environmental Toxicology, Guangxi University, Nanning, People's Republic of China.
J Sep Sci. 2016 Sep;39(18):3638-46. doi: 10.1002/jssc.201600614. Epub 2016 Aug 25.
Analytical methods based on multiplug filtration cleanup coupled with pulse glow discharge-ion mobility spectrometry and liquid chromatography tandem mass spectrometry were developed for the analysis of tricaine mesylate residue in fish and fish-raising water samples. A silica fiber holder and an appropriate new interface were designed to make the direct introduction of the fiber into the pulse glow discharge-ion mobility spectrometry introduction mechanism. The multiplug filtration cleanup method with adsorption mixtures was optimized for the determination of tricaine mesylate in fish samples. Good linear relationships were obtained by the two methods. For fish samples, limits of detection were 6 and 0.6 μg/kg by ion mobility spectrometry and liquid chromatography with tandem mass spectrometry, respectively. The matrix effect of the established liquid chromatography tandem mass spectrometry method was negligible for fish samples but that of the ion mobility spectrometry method was not. The two methods were compared. The ion mobility spectrometry system could be used a rapid screening tool on site with the advantage of rapidity, simplicity, and portability, and the liquid chromatography tandem mass spectrometry system could be used for validation in laboratory conditions with the advantage of lower limit of detection, stability, and precision.
建立了基于多插头过滤净化结合脉冲辉光放电-离子迁移谱和液相色谱串联质谱的分析方法，用于分析鱼及养鱼水样中甲磺酸三卡因残留。设计了一种石英纤维支架和一个合适的新型接口，以便将纤维直接引入脉冲辉光放电-离子迁移谱进样装置。对采用吸附混合物的多插头过滤净化方法进行了优化，用于测定鱼样中的甲磺酸三卡因。两种方法均获得了良好的线性关系。对于鱼样，离子迁移谱法和液相色谱串联质谱法的检测限分别为6和0.6 μg/kg。所建立的液相色谱串联质谱法对鱼样的基质效应可忽略不计，但离子迁移谱法的基质效应不可忽略。对这两种方法进行了比较。离子迁移谱系统可作为现场快速筛查工具，具有快速、简便和便携的优点，而液相色谱串联质谱系统可在实验室条件下用于验证，具有检测限低、稳定性和精密度高的优点。
