Xie Chaonan, Li Qin, Han Gang, Liu Huan, Yang Jien, Li Jincheng
Chinese Academy of Fishery Sciences, Beijing, P. R. China.
Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing, P. R. China.
Biomed Chromatogr. 2019 May;33(5):e4512. doi: 10.1002/bmc.4512. Epub 2019 Mar 6.
Tricaine methanesulfonate is one of most commonly used anesthetics in fish during blood sampling, artificial propagation and long-distance transportation. In this study, an accurate method for the quantitative determination of tricaine in fish samples by a stable isotope dilution assay coupled with high-performance liquid chromatography-triple quadrupole mass spectrometry was developed. Tricaine-D was synthesized and used as an isotopically labeled internal standard for the determination of tricaine. The analytical performance of the method was validated for tricaine determination in marine fish and freshwater fish. The determination of tricaine was linear in the range of 2.0-200.0 μg L . The limit of detection and limit of quantitation for fish muscle tissues were 1.0 and 4.0 μg kg , respectively. Good recoveries were obtained in the range of 92.08-97.50%. The inter- and intra-assay relative standard deviations (RSD values) were investigated, and the values were 0.39-3.01 and 0.85-2.77%, respectively. The values of CCα and CCβ were 10.21-10.43 and 10.42-10.87 μg kg , respectively. The clearance of MS-222 from grass carp was further studied using our method. The results demonstrate that MS-222 could be well absorbed and rapidly eliminated after bath administration.
甲磺酸三卡因是鱼类在采血、人工繁殖和长途运输过程中最常用的麻醉剂之一。在本研究中，建立了一种通过稳定同位素稀释法结合高效液相色谱 - 三重四极杆质谱法定量测定鱼样品中甲磺酸三卡因的准确方法。合成了甲磺酸三卡因 - D并用作测定甲磺酸三卡因的同位素标记内标。该方法的分析性能在海水鱼和淡水鱼中甲磺酸三卡因的测定中得到验证。甲磺酸三卡因的测定在2.0 - 200.0μg/L范围内呈线性。鱼肌肉组织的检测限和定量限分别为1.0和4.0μg/kg。回收率在92.08 - 97.50%范围内良好。研究了批间和批内相对标准偏差（RSD值），其值分别为0.39 - 3.01%和0.85 - 2.77%。CCα和CCβ的值分别为10.21 - 10.43和10.42 - 10.87μg/kg。使用我们的方法进一步研究了草鱼体内甲磺酸三卡因的清除情况。结果表明，甲磺酸三卡因经浸浴给药后可被良好吸收并迅速消除。
