Manyak M J, Nelson L M, Solomon D, Russo A, Thomas G F, Stillman R J
Radiation Oncology Branch, National Cancer Institute, Bethesda, Maryland.
Fertil Steril. 1989 Jul;52(1):140-5. doi: 10.1016/s0015-0282(16)60803-8.
The potential of photodynamic therapy for endometriosis was evaluated by autotransplantation of endometrial tissue in 15 female virgin New Zealand white rabbits. After maturation, 14 animals were injected intravenously with 10 mg/kg of dihematoporphyrin ether (DHE) and, 24 hours later, transplants were exposed to 630 nm light at 100 to 210 mW/cm2. Sixteen transplants received 100 J/cm2, 10 transplants received 50 J/cm2; 13 untreated transplants served as controls. In the remaining animal that did not receive DHE, 3 transplants were irradiated with 100 J/cm2. Six days after treatment, transplants were harvested with the underlying musculature, and multiple sections were examined histopathologically. Complete endometrial epithelial destruction was seen in 13 of 16 transplants (81%) in the 100 J/cm2 group and in 6 of 10 transplants (60%) in the 50-J/cm2 group. Nearly complete endometrial epithelial destruction was seen in 2 other transplants in each group. No damage occurred in either the 3 transplants that received 100 J/cm2 without prior DHE or in the 13 transplants with DHE and no irradiation. The sensitivity of ectopic rabbit endometrial tissue encourages further evaluation of photodynamic therapy for the treatment of human endometrial disorders.
通过在15只雌性新西兰纯种白兔身上进行子宫内膜组织自体移植，评估了光动力疗法治疗子宫内膜异位症的潜力。在移植组织成熟后，14只动物静脉注射10mg/kg的二血卟啉醚（DHE），24小时后，将移植组织暴露于波长630nm、强度为100至210mW/cm2的光线下。16个移植组织接受100J/cm2的光照，10个移植组织接受50J/cm2的光照；13个未处理的移植组织作为对照。在其余未接受DHE的动物身上，3个移植组织接受了100J/cm2的光照。治疗6天后，将移植组织及其下方的肌肉组织一并切除，制作多个切片进行组织病理学检查。在接受100J/cm2光照的16个移植组织中，有13个（81%）出现了子宫内膜上皮的完全破坏；在接受50J/cm2光照的10个移植组织中，有6个（60%）出现了这种情况。每组另外还有2个移植组织出现了近乎完全的子宫内膜上皮破坏。在未预先使用DHE而接受100J/cm2光照的3个移植组织以及使用了DHE但未接受光照的13个移植组织中，均未出现损伤。异位兔子宫内膜组织的敏感性促使人们进一步评估光动力疗法在治疗人类子宫内膜疾病方面的效果。
