Wyss P, Steiner R, Liaw L H, Wyss M T, Ghazarians A, Berns M W, Tromberg B J, Tadir Y
Beckman Laser Institute and Medical Clinic, Department of Obstetrics and Gynecology, University of California, Irvine, USA.
Hum Reprod. 1996 Sep;11(9):1992-7. doi: 10.1093/oxfordjournals.humrep.a019531.
The origin and process of regeneration in rabbit endometrium was evaluated following photodynamic epithelial destruction using topically applied aminolevulinic acid (ALA). Selective destruction of endometrial epithelium was performed using photodynamic therapy (PDT). ALA was diluted to 200 mg/ml dextran 70 shortly prior to administration. A volume of 1.2 ml was injected into the left uterus. Intrauterine illumination (wavelength 630 nm, light dose 40-80 J/cm2) was performed 3 h after drug administration. Tissue morphology was evaluated by light and scanning electron microscopy 1, 3, 7 and 28 days post-treatment (three animals at each time-point). Regeneration of the endometrium following epithelial ablation by PDT was fully activated after 24 h and was completed after 72 h. Endometrial surface generation occurred by proliferation, originating primarily in deeper regions of the glands. Findings from our morphological follow-up study support the origin of endometrial regeneration being mainly from undifferentiated stem cells and residual glandular epithelium.
使用局部应用的氨基乙酰丙酸（ALA）进行光动力上皮破坏后，评估了兔子宫内膜再生的起源和过程。采用光动力疗法（PDT）对子宫内膜上皮进行选择性破坏。给药前不久，将ALA稀释至200 mg/ml葡聚糖70。向左侧子宫内注入1.2 ml。给药3小时后进行子宫内照射（波长630 nm，光剂量40 - 80 J/cm²）。在治疗后1、3、7和28天通过光学显微镜和扫描电子显微镜评估组织形态（每个时间点三只动物）。PDT上皮消融后，子宫内膜再生在24小时后完全激活，并在72小时后完成。子宫内膜表面通过增殖产生，主要起源于腺体的较深区域。我们形态学随访研究的结果支持子宫内膜再生主要源于未分化干细胞和残留腺上皮。
