具有不同DNA模板引物结合亲和力和不同比活性的诺曼鼠肉瘤DNA聚合酶α形式的纯化。

Purification of Norman Murine Sarcoma DNA polymerase alpha forms with different DNA template primer binding affinity and different specific activity.

Sylvia V L, Norman J O, Curtin G M, Stec J, Busbee D L

Department of Anatomy, College of Veterinary Medicine, Texas A&M University, College Station 77843.

Int J Biochem. 1989;21(2):203-8. doi: 10.1016/0020-711x(89)90110-9.

Abstract

DNA polymerase alpha was isolated from Norman Murine Myxosarcoma cells using ion exchange, immunoaffinity, and DNA affinity chromatography, showing two distinct enzyme forms designated A1 and A2. 2. Chromatographic analysis of polymerase alpha forms A1 and A2 indicate a charge difference and a difference in affinity of binding to DNA between polymerase alpha forms which were equally reactive to anti-DNA polymerase alpha monoclonal IgG. 3. Polymerase A1 specific activity was about 3600 U/mg while A2 specific activity was about 40,000 U/mg.

摘要

使用离子交换、免疫亲和及DNA亲和色谱法从诺曼氏小鼠黏液肉瘤细胞中分离出DNA聚合酶α，其呈现出两种不同的酶形式，分别命名为A1和A2。2. 对聚合酶α的A1和A2形式进行色谱分析表明，这两种形式之间存在电荷差异以及与DNA结合亲和力的差异，它们对抗DNA聚合酶α单克隆IgG的反应性相同。3. 聚合酶A1的比活性约为3600 U/mg，而A2的比活性约为40,000 U/mg。