Smith M J, Banfield D K, Doteval K, Gorski S, Kowbel D J
Institute of Molecular Biology and Biochemistry, Department of Biological Sciences, Simon Fraser University, Burnaby, BC, Canada.
Gene. 1989 Mar 15;76(1):181-5. doi: 10.1016/0378-1119(89)90022-x.
The mitochondrial (mt) DNA from the sea star Pisaster ochraceus has been isolated, restriction-mapped, and cloned into plasmid vectors. Both ribosomal RNA genes, the genes for 12 of the 13 mitochondrial proteins, and 11 of the tRNA genes have been localized by DNA sequence analyses. The sequence arrangement of the genes is markedly different from that seen in sea urchin mitochondrial DNA. A segment of the DNA molecule extending from tRNA(pro), including the tRNA cluster, ND1, ND2, and 16S genes, is inverted in relation to the sea urchin genome. The resulting gene order in the sea star is 12S, 16S, ND2, tRNA cluster, COI. As a result of the inversion, the transcriptional polarity of ND1, ND2, and 16S genes are opposite to that of the 12S and COI genes. The arrangement and transcriptional polarity of the other genes mapped here is the same as seen in urchin.
海星赭色海星的线粒体（mt）DNA已被分离、进行了限制性图谱分析，并克隆到质粒载体中。通过DNA序列分析确定了两个核糖体RNA基因、13种线粒体蛋白中的12种基因以及11种tRNA基因的位置。这些基因的序列排列与海胆线粒体DNA中的明显不同。从tRNA（pro）延伸的一段DNA分子，包括tRNA簇、ND1、ND2和16S基因，相对于海胆基因组是倒置的。海星中由此产生的基因顺序是12S、16S、ND2、tRNA簇、COI。由于这种倒置，ND1、ND2和16S基因的转录极性与12S和COI基因的相反。这里绘制的其他基因的排列和转录极性与海胆中的相同。
