Dragoi Ioan-Catalin, Stanciu Stefan G, Hristu Radu, Coanda Henri-George, Tranca Denis E, Popescu Marius, Coltuc Dinu
Electrical Engineering Department, Valahia University of Targoviste, Bd. Carol I, nr. 2, Targoviste, Romania.
Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei Bvd., Bucharest, Romania;
Biomed Opt Express. 2016 Mar 3;7(4):1127-37. doi: 10.1364/BOE.7.001127. eCollection 2016 Apr 1.
Complementary laser scanning microscopy micrographs are considered as pairs consisting in a master image (MI) and a slave image (SI), the latter with potential for facilitating the interpretation of the MI. We propose a strategy based on reversible watermarking for embedding a lossy compressed version of the SI into the MI. The use of reversible watermarking ensures the exact recovery of the host image. By storing and/or transmitting the watermarked MI in a single file, the information contained in both images that constitute the pair is made available to a potential end-user, which simplifies data association and transfer. Examples are presented using support images collected by two complementary techniques, confocal scanning laser microscopy and transmission laser scanning microscopy, on Hematoxylin and Eosin stained tissue fragments. A strategy for minimizing the watermarking distortions of the MI, while preserving the content of the SI, is discussed in detail.
互补激光扫描显微镜图像被视为由主图像（MI）和从图像（SI）组成的对，后者有助于对主图像的解释。我们提出了一种基于可逆水印的策略，用于将从图像的有损压缩版本嵌入主图像中。使用可逆水印可确保宿主图像的精确恢复。通过将水印主图像存储和/或传输到单个文件中，构成该对的两个图像中包含的信息可供潜在的最终用户使用，这简化了数据关联和传输。给出了使用通过共聚焦扫描激光显微镜和透射激光扫描显微镜这两种互补技术收集的苏木精和伊红染色组织切片的支持图像的示例。详细讨论了一种在保留从图像内容的同时最小化主图像水印失真的策略。
