Fairhead A P, Brooks S G, Butterworth K R, Mangham B A
Department of Clinical Chemistry, Rorer Health Care Limited, Eastbourne, East Sussex.
Food Chem Toxicol. 1989 May;27(5):341-5. doi: 10.1016/0278-6915(89)90137-3.
An automated trace enrichment method for metoclopramide is described. Serum was injected on to a short column packed with PLRP-S 100A (a polymeric reversed-phase material). The unwanted components were washed off with borate buffer (pH 9.8) before switching to a Spherisorb ODS column for the separation. The limit of detection in serum samples was 2 ng/ml. The method was used in a pharmacokinetic study to compare the biovailability of two formulations of the drug.
描述了一种用于甲氧氯普胺的自动痕量富集方法。将血清注入填充有PLRP - S 100A（一种聚合反相材料）的短柱上。在切换到Spherisorb ODS柱进行分离之前，用硼酸盐缓冲液（pH 9.8）洗去不需要的成分。血清样品中的检测限为2 ng/ml。该方法用于一项药代动力学研究，以比较该药物两种制剂的生物利用度。
