一种用于测定血清中胃复安的自动化高效液相色谱痕量富集方法及其在人体生物等效性志愿者研究中的应用。
An automated high-performance liquid chromatographic trace enrichment method for the determination of metoclopramide in serum and its application to a bioequivalence human volunteer study.
作者信息
Fairhead A P, Brooks S G, Butterworth K R, Mangham B A
机构信息
Department of Clinical Chemistry, Rorer Health Care Limited, Eastbourne, East Sussex.
出版信息
Food Chem Toxicol. 1989 May;27(5):341-5. doi: 10.1016/0278-6915(89)90137-3.
An automated trace enrichment method for metoclopramide is described. Serum was injected on to a short column packed with PLRP-S 100A (a polymeric reversed-phase material). The unwanted components were washed off with borate buffer (pH 9.8) before switching to a Spherisorb ODS column for the separation. The limit of detection in serum samples was 2 ng/ml. The method was used in a pharmacokinetic study to compare the biovailability of two formulations of the drug.
描述了一种用于甲氧氯普胺的自动痕量富集方法。将血清注入填充有PLRP - S 100A（一种聚合反相材料）的短柱上。在切换到Spherisorb ODS柱进行分离之前，用硼酸盐缓冲液（pH 9.8）洗去不需要的成分。血清样品中的检测限为2 ng/ml。该方法用于一项药代动力学研究，以比较该药物两种制剂的生物利用度。
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