番茄叶片感染后乙烯生成的定量分析 。 你提供的原文似乎不完整，“Infected by.”后面缺少具体内容。
Quantification of Ethylene Production in Tomato Leaves Infected by .
作者信息
Kim Jung-Gun, Stork William, Mudgett Mary Beth
机构信息
Department of Biology, Stanford University, Stanford, USA.
出版信息
Bio Protoc. 2016 Feb 5;6(3). doi: 10.21769/bioprotoc.1723.
Ethylene is a gaseous plant hormone controlling fruit ripening, flower opening, leaf senescence as well as abscission, and disease symptom development. Ethylene plays a critical role in the bacterial pathogen ()-elicited symptom development in tomato. This protocol describes the measurement of ethylene gas produced by tomato leaves infected with . Infected leaflets are placed in a glass tube for 30 min without sealing. The glass tubes are then capped with a septa stopper, and incubated for an hour. A 1 ml gas sample is removed from the tube using a syringe and then injected into a gas chromatograph to quantify ethylene gas levels. This protocol will be applicable for other plants with other pathogens with modifications.
乙烯是一种气态植物激素，可控制果实成熟、花朵开放、叶片衰老以及脱落，还能影响疾病症状的发展。乙烯在细菌病原体引发的番茄症状发展过程中起着关键作用。本方案描述了感染病原体的番茄叶片所产生乙烯气体的测量方法。将感染的小叶置于玻璃管中30分钟，不进行密封。然后用隔垫塞封住玻璃管，并孵育一小时。使用注射器从管中抽取1毫升气体样本，然后注入气相色谱仪以量化乙烯气体水平。经修改后，本方案将适用于感染其他病原体的其他植物。
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