Lu You-Jin, Liu Rong-Yu, Hu Kun, Wang Ying
Department of Respiratory Medicine, The Second Affiliated Hospital of Anhui Medical University, Hefei, 230032, Anhui, China.
Department of Geriatric Respiratory Medicine, The First Affiliated Hospital of Anhui Medical University, 218 Jixi Road, Hefei, 230022, Anhui, China.
Tumour Biol. 2016 Sep;37(9):12685-12695. doi: 10.1007/s13277-016-5241-5. Epub 2016 Jul 22.
Lung cancer remains a leading cause of cancer-associated mortality worldwide, and non-small lung cancer (NSCLC) is responsible for over 80 % of lung cancer-related deaths. Identifying novel molecular biomarker that can inhibit the progression of lung cancer will facilitate the development of new treatment strategies. Herein, we demonstrated that miR-541-3p is a tumor-suppressor microRNA (miRNA) in NSCLC progression. We found that expression of miR-541-3p was decreased obviously in NSCLC tissues and plasma. Down-regulation of miR-541-3p was associated with TNM stage and postoperative survival. Overexpression of miR-541-3p inhibited the growth and metastasis of NSCLC cells. The TGIF2 was a direct target of miR-541-3p and promoted the growth and metastasis of NSCLC cells. Further study showed that TGIF2 could reverse the inhibitory effect of miR-541-3p on growth and metastasis of NSCLC cells. Taken together, our data highlight the pivotal role of miR-541-3p in the progression of NSCLC. Thus, miR-541-3p may be a potential prognostic marker and of treatment relevance for NSCLC progression intervention.
肺癌仍然是全球癌症相关死亡的主要原因，其中非小细胞肺癌（NSCLC）占肺癌相关死亡的80%以上。鉴定能够抑制肺癌进展的新型分子生物标志物将有助于开发新的治疗策略。在此，我们证明了miR-541-3p在NSCLC进展中是一种肿瘤抑制性微小RNA（miRNA）。我们发现miR-541-3p在NSCLC组织和血浆中的表达明显降低。miR-541-3p的下调与TNM分期和术后生存率相关。miR-541-3p的过表达抑制了NSCLC细胞的生长和转移。TGIF2是miR-541-3p的直接靶点，并促进NSCLC细胞的生长和转移。进一步研究表明，TGIF2可以逆转miR-541-3p对NSCLC细胞生长和转移的抑制作用。综上所述，我们的数据突出了miR-541-3p在NSCLC进展中的关键作用。因此，miR-541-3p可能是一种潜在的预后标志物，与NSCLC进展干预的治疗相关。
