Calvo-Polanco Monica, Sánchez-Castro Iván, Cantos Manuel, García José Luis, Azcón Rosario, Ruiz-Lozano Juan Manuel, Beuzón Carmen R, Aroca Ricardo
Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín (CSIC), C/Profesor Albareda, Granada, 18008, Spain.
Biochimie et Physiologie Moléculaire des Plantes, SupAgro/INRA UMR 5004. 2, Place Viala, Montpellier, Cedex 2 34060, France.
Plant Cell Environ. 2016 Nov;39(11):2498-2514. doi: 10.1111/pce.12807. Epub 2016 Oct 3.
The adaptation capacity of olive trees to different environments is well recognized. However, the presence of microorganisms in the soil is also a key factor in the response of these trees to drought. The objective of the present study was to elucidate the effects of different arbuscular mycorrhizal (AM) fungi coming from diverse soils on olive plant growth and water relations. Olive plants were inoculated with native AM fungal populations from two contrasting environments, that is, semi-arid - Freila (FL) and humid - Grazalema (GZ) regions, and subjected to drought stress. Results showed that plants grew better on GZ soil inoculated with GZ fungi, indicating a preference of AM fungi for their corresponding soil. Furthermore, under these conditions, the highest AM fungal diversity was found. However, the highest root hydraulic conductivity (Lp ) value was achieved by plants inoculated with GZ fungi and growing in FL soil under drought conditions. So, this AM inoculum also functioned in soils from different origins. Nine novel aquaporin genes were also cloned from olive roots. Diverse correlation and association values were found among different aquaporin expressions and abundances and Lp , indicating how the interaction of different aquaporins may render diverse Lp values.
油橄榄树对不同环境的适应能力已得到广泛认可。然而，土壤中微生物的存在也是这些树木应对干旱的关键因素。本研究的目的是阐明来自不同土壤的不同丛枝菌根（AM）真菌对油橄榄植株生长和水分关系的影响。用油橄榄植株接种来自两个截然不同环境（即半干旱的弗雷拉（FL）地区和湿润的格拉察莱马（GZ）地区）的本地AM真菌种群，并使其遭受干旱胁迫。结果表明，接种GZ真菌的植株在GZ土壤上生长得更好，这表明AM真菌对其相应的土壤具有偏好性。此外，在这些条件下，发现了最高的AM真菌多样性。然而，在干旱条件下，接种GZ真菌并生长在FL土壤中的植株实现了最高的根水力导度（Lp）值。所以，这种AM接种剂在不同来源的土壤中也发挥了作用。还从油橄榄根中克隆出了9个新的水通道蛋白基因。在不同水通道蛋白的表达、丰度与Lp之间发现了不同的相关性和关联值，这表明不同水通道蛋白之间的相互作用可能产生不同的Lp值。
