Onorati Michela, Sancesario Giulia, Pastore Donatella, Bernardini Sergio, Carrión Jorge E, Carosi Monica, Vignoli Leonardo, Lauro Davide, Gentile Gabriele
Department of Science, University Roma Tre, Rome, Italy; Department of Biology, University of Rome Tor Vergata, Italy.
Department of Clinical and Behavioral Neurology IRCC S. Lucia-Rome, Italy.
Anim Reprod Sci. 2016 Sep;172:105-13. doi: 10.1016/j.anireprosci.2016.07.007. Epub 2016 Jul 15.
In a combined approach, endocrine and ultrasonic analyses were performed to assess reproduction of two syntopic populations of terrestrial Galápagos iguanas the Conolophus marthae (the Galápagos Pink Land Iguana) and C. subcristatus on the Volcán Wolf (Isabela Island). The ELISA methods (enzyme-linked immunosorbent assay) were used to measure plasma concentrations of progesterone (P4) and 17β-estradiol (E2) from samples collected over the course of three different seasons: July 2010, June 2012-2014. As for C. subcristatus, the large number of females with eggs in 2012 and 2014 were associated with increased plasma P4 concentrations and the corresponding absence of females with eggs in July 2010 when concentrations of both hormones levels were basal indicating reproduction was still ongoing in June and had ended in July. In C. marthae, even though there was a positive relationship between egg-development stages and hormone concentrations, P4 concentrations were basal through the three years that samples were collected, with some females having a lesser number of eggs compared with C. subcristatus. In C. marthae P4 and E2 patterns did not allow for defining a specific breeding season.
采用综合方法，对加拉帕戈斯陆鬣蜥的两个同域种群——沃尔夫火山（伊莎贝拉岛）上的马尔塔陆鬣蜥（加拉帕戈斯粉红陆鬣蜥）和康氏陆鬣蜥进行了内分泌和超声分析，以评估其繁殖情况。采用酶联免疫吸附测定法（ELISA），对在三个不同季节（2010年7月、2012年6月至2014年）采集的样本中的孕酮（P4）和17β-雌二醇（E2）血浆浓度进行测量。对于康氏陆鬣蜥，2012年和2014年大量怀有卵的雌性与血浆P4浓度升高有关，而2010年7月怀有卵的雌性不存在，当时两种激素水平均处于基础水平，表明繁殖在6月仍在进行，7月已经结束。对于马尔塔陆鬣蜥，尽管卵发育阶段与激素浓度之间存在正相关关系，但在采集样本的三年中P4浓度一直处于基础水平，与康氏陆鬣蜥相比，一些雌性的卵数量较少。对于马尔塔陆鬣蜥，P4和E2模式无法确定一个特定的繁殖季节。
