Kalenov Sergei V, Baurina Marina M, Skladnev Dmitry A, Kuznetsov Alexander Ye
The Department of Biotechnology, Faculty of Biotechnology and Industrial Ecology, D.I. Mendeleyev University of Chemical Technology of Russia, 9 Miusskaya square, Moscow 125047, Russia.
The Department of Biotechnology, Faculty of Biotechnology and Industrial Ecology, D.I. Mendeleyev University of Chemical Technology of Russia, 9 Miusskaya square, Moscow 125047, Russia.
J Biotechnol. 2016 Sep 10;233:211-8. doi: 10.1016/j.jbiotec.2016.07.014. Epub 2016 Jul 19.
Submerged growth of Halobacterium salinarum and therefore synthesis of bacteriorhodopsin (BR) and carotenoids depend greatly on products of both chemical and/or photochemical oxidation of medium components and cellular metabolism which act as inhibitors. Some cultivation variants which allowed eliminating an adverse effect of inhibitors on biomass accumulation and BR synthesis are reviewed. The application of activated charcoal or ion exchange resin as adsorbents at preparing inoculums and the main cultivation stages was shown to allow controlling, namely lowering overstress of the halobacterial cells by metabolites. The halobacterial biomass containing BR up to 1,750mgL(-1) and the minimum amount of carotinoids that would BR greatly facilitate isolation was accumulated up to 45gL(-1) during eight-day cultivation with cell recycling through adsorbent suspension in a fed-batch mode. To control BR biosynthesis the express method of BR quantification based on colour shades of cell suspension was developed.
嗜盐菌（Halobacterium salinarum）的 submerged growth 以及因此视紫红质（BR）和类胡萝卜素的合成在很大程度上取决于培养基成分的化学和/或光化学氧化产物以及作为抑制剂的细胞代谢产物。本文综述了一些能够消除抑制剂对生物量积累和 BR 合成不利影响的培养变体。研究表明，在制备接种物和主要培养阶段使用活性炭或离子交换树脂作为吸附剂，可以控制，即降低代谢产物对嗜盐菌细胞的过度压力。在补料分批培养模式下，通过吸附剂悬浮液进行细胞循环，在为期八天的培养过程中，积累了高达 45gL(-1)的含有高达 1750mgL(-1)BR 且类胡萝卜素含量最低（这将极大地促进 BR 的分离）的嗜盐菌生物量。为了控制 BR 的生物合成，开发了基于细胞悬浮液颜色深浅的 BR 定量快速方法。
