Kalenov Sergei V, Gordienko Mariia G, Murzina Ekaterina D, Poberezhniy Daniil Y, Baurin Dmitry V, Suzina Natalia E, Morozov Alexander N, Yakubovich Liubov M, Belov Alexey A, Panfilov Victor I, Yarovaya Oksana V, Il'in Michail M, Sorokin Vladimir V, Skladnev Dmitry A
Department of Biotechnology, Faculty of Biotechnology and Industrial Ecology, D.I. Mendeleyev University of Chemical Technology of Russia, 9 Miusskaya square, Moscow, 125047, Russia.
Department of Cybernetics, Faculty of Computer Aided Process Engineering, Mendeleyev University of Chemical Technology of Russia, 9 Miusskaya square, Moscow, Russia.
Extremophiles. 2018 May;22(3):511-523. doi: 10.1007/s00792-018-1013-z. Epub 2018 Feb 17.
Spray drying is appropriate for the preservation of halophilic microorganisms due to the nature of these microorganisms, as they survive in adverse environmental conditions by being encapsulated in salt crystals. Artificial neural networks were in this study used to optimize practically significant spray-drying regimes of the C-carotenoids producer Halobacterium salinarum. Immediately after drying, the samples contained up to 54% halobacterial biomass and less than 5% moisture, and the level of preservation of carotenoids was 95-97%. The storage of biomass at 4 °C resulted in the gradual degradation of the carotenoids, which reached 58-64% in the best samples after 1 year. A comprehensive study of changes in halobacteria biomass after spray drying and the nature of the damage provided new data on the survival and preservation of cells and biologically active substances in the various spray-drying regimes and at different storage times.
由于嗜盐微生物的特性，喷雾干燥适合用于保存这类微生物，因为它们通过被包裹在盐晶体中在不利的环境条件下存活。在本研究中，人工神经网络被用于优化类胡萝卜素生产者盐生盐杆菌实际可行的喷雾干燥方案。干燥后立即测定，样品中含有高达54%的盐杆菌生物量和低于5%的水分，类胡萝卜素的保存率为95 - 97%。在4℃下储存生物量导致类胡萝卜素逐渐降解，1年后最佳样品中的降解率达到58 - 64%。对喷雾干燥后盐杆菌生物量变化及损伤性质的综合研究，为不同喷雾干燥方案和不同储存时间下细胞及生物活性物质的存活和保存提供了新的数据。
