Lotfalah Moradi Sadegh, Hajishafieeha Zahra, Nojedehi Shahrzad, Dinarvand Vida, Hesami Tackallou Saeed, Roy Ram V, Ardeshirylajimi Abdolreza, Soleimani Masoud
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Cell Culture Media, Danesh Azma Cell Company, Tehran, Iran.
Department of Microbiology, Qazvin University of Medical Sciences, Qazvin, Iran.
Biologicals. 2016 Sep;44(5):387-93. doi: 10.1016/j.biologicals.2016.05.007. Epub 2016 Jul 20.
Induced pluripotent stem cells (iPSCs) are unique and unlimited clinical sources of stem cell therapy for the regenerative medicine. Feeder layer preparation is an important step for iPSCs production, which is expensive, time-consuming and requires conversance. In the present study, we investigated the maintenance of pluripotency, and stemness of the iPSCs through feeder-free culture on a collagen-grafted Mixed Cellulose Esters membrane (MCE-COL) after three passages during twelve days. Results have demonstrated that the iPSCs cultured on MCE-COL membrane had a fine, typical undifferentiated morphology, increased proliferation rate and significant multi-lineage differentiation potential. Alkaline phosphatase (ALP) staining and pluripotency associated gene markers expression further confirmed that iPSCs cultured on the surface of MCE-COL had more ALP positive colonies and enhanced expression of Oct-4, Nanog, Sox-2 and ALP in comparison with MCE and control groups. Since MCE-COL membrane has three dimensional structure and bioactivity, it has the potential for usage in the feeder-free culture of iPSCs, and could be a suitable candidate to use as a feeder layer in stem cells preparation.
