Siqueira Halmisson D'Arley S, Neto Benedito S, Sousa Damião P, Gomes Bruno S, da Silva Francilene Vieira, Cunha Francisco V M, Wanderley Carlos W S, Pinheiro Gabriel, Cândido André G F, Wong Deysi V T, Ribeiro Ronaldo A, Lima-Júnior Roberto C P, Oliveira Francisco A
Medicinal Plants Research Center, Federal University of Piauí, (unnumbered), 64049-550 Teresina, Piauí, Brazil; Physiology and Pharmacology Department, Medicine Faculty, Federal University of Ceará, Cel. Nunes de Melo 1127, 60430-270 Fortaleza, CE, Brazil.
Pharmaceutical Sciences Department, Federal University of Paraíba, Jardim Universitário, 58051-970 João Pessoa, PB, Brazil; Physiology and Pharmacology Department, Medicine Faculty, Federal University of Ceará, Cel. Nunes de Melo 1127, 60430-270 Fortaleza, CE, Brazil.
Life Sci. 2016 Sep 1;160:27-33. doi: 10.1016/j.lfs.2016.07.008. Epub 2016 Jul 20.
We aimed to investigate the modulating effect of α-phellandrene on neutrophil migration and mast cell degranulation processes.
Male Wistar rats or Swiss mice were treated p.o. with vehicle (3% Tween 80, p.o.), α-phellandrene (50, 100, or 200mg/kg, p.o.), or dexamethasone (0.5mg/kg, p.o.) 1h before carrageenan injection. Then, the neutrophil migration in 6-day-old air pouches or peritoneal cavities. The leukocyte rolling and adhesion were measured in real time and assessed by intravital microscopy. ELISA was used to detect TNF-α and IL-6 in peritoneal lavage. Compound 48/80-induced mast cell degranulation was assessed in mesenteric rat tissues.
In all the tested doses, α-phellandrene prevented carrageenan-induced neutrophil accumulation (P<0.05). As detected by intravital microscopy, α-phellandrene also inhibited leukocyte rolling and adhesion, as well as significantly inhibited the production of the pro-inflammatory cytokines TNF-α and IL-6. Moreover, the degranulation of compound 48/80-induced mast cells was also inhibited by α-phellandrene (P<0.001).
These results suggest that α-phellandrene plays an important role as an anti-inflammatory agent through neutrophil migration modulation and mast cell stabilization.
我们旨在研究α-水芹烯对中性粒细胞迁移和肥大细胞脱颗粒过程的调节作用。
在注射角叉菜胶前1小时，给雄性Wistar大鼠或瑞士小鼠经口灌胃给予赋形剂（3%吐温80，经口）、α-水芹烯（50、100或200mg/kg，经口）或地塞米松（0.5mg/kg，经口）。然后，检测6日龄气袋或腹腔内的中性粒细胞迁移情况。通过活体显微镜实时测量白细胞滚动和黏附，并进行评估。采用酶联免疫吸附测定法检测腹腔灌洗液中的肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）。在大鼠肠系膜组织中评估化合物48/80诱导的肥大细胞脱颗粒情况。
在所有测试剂量下，α-水芹烯均可预防角叉菜胶诱导的中性粒细胞聚集（P<0.05）。通过活体显微镜检测发现，α-水芹烯还可抑制白细胞滚动和黏附，并显著抑制促炎细胞因子TNF-α和IL-6的产生。此外，α-水芹烯还可抑制化合物48/80诱导的肥大细胞脱颗粒（P<0.001）。
这些结果表明，α-水芹烯通过调节中性粒细胞迁移和稳定肥大细胞，作为一种抗炎剂发挥重要作用。
