通过二维水平电泳的简单方法检测到家狐中三种新的血浆蛋白多态性。

Three new plasma protein polymorphisms in domestic foxes, detected by a simple method of 2D horizontal electrophoresis.

作者信息

Juneja R K, Niini T, Larsson H E, Gahne B

出版信息

Hereditas. 1989;110(2):159-64. doi: 10.1111/j.1601-5223.1989.tb00436.x.

DOI:10.1111/j.1601-5223.1989.tb00436.x

Abstract

Plasma samples of 270 foxes from 45 complete families (14 of arctic foxes, 28 of silver foxes and 3 with arctic x silver fox hybrid offspring) were analysed by a method of two-dimensional agarose gel (pH 5.4)-horizontal polyacrylamide gel (pH 9.0) electrophoresis followed by general-protein staining of gels. Genetic polymorphism of three plasma proteins, tentatively designated prealbumin (Pr), postalbumin 1 (Pa1), and pretransferin 1 (Prt1), was observed. In silver foxes, Pa1 and Prt1 showed a high degree of polymorphism, each with 3 common alleles, while Pr showed a scarce polymorphism. The arctic foxes were monomorphic for Pr and Prt1 and showed a scarce Pa1 polymorphism. The Prt1 phenotype of arctic foxes showed identical mobility with one of the Prt1 variants of silver foxes.

摘要

采用二维琼脂糖凝胶（pH 5.4）-水平聚丙烯酰胺凝胶（pH 9.0）电泳法，随后对凝胶进行总蛋白染色，分析了来自45个完整家庭的270只狐狸的血浆样本（14只北极狐、28只银狐和3只北极狐与银狐杂交后代）。观察到三种血浆蛋白的遗传多态性，暂定为前白蛋白（Pr）、后白蛋白1（Pa1）和前转铁蛋白1（Prt1）。在银狐中，Pa1和Prt1表现出高度多态性，各有3个常见等位基因，而Pr表现出罕见的多态性。北极狐的Pr和Prt1是单态的，Pa1多态性较少。北极狐的Prt1表型与银狐的一种Prt1变体具有相同的迁移率。

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