Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS-CSIC), Apdo. 1052, 41080 Seville, Spain.
Departamento de Cristalografía, Mineralogía y Química Agrícola, Escuela Técnica Superior de Ingeniería Agronómica, Universidad de Sevilla, Ctr. Utrera km. 1, 41013 Seville, Spain.
Sci Total Environ. 2016 Nov 15;571:42-9. doi: 10.1016/j.scitotenv.2016.07.110. Epub 2016 Jul 23.
The objectives of this study were to investigate the mutual effect of the PAHs fluorene and pyrene on their respective biodegradation and dissipation processes in an agricultural soil, and to determine the effect of hydroxypropyl-β-cyclodextrin (HPBCD), used to increase the bioavailability of PAHs, on such processes. Fluorene dissipation was primarily due to abiotic processes, although a small contribution from biodegradation was also observed. Therefore, fluorene dissipation did not increase with HPBCD and its presence did not significantly alter the dehydrogenase activity. In contrast to fluorene, pyrene dissipation depended primarily on biotic factors, with endogenous soil microorganisms capable of degrading pyrene, with large increases in dehydrogenase activity. HPBCD increased biodegradation rate of pyrene. The co-contamination of soil with both PAHs did not affect fluorene evolution, but significantly inhibited pyrene biodegradation. The different abilities of soil bacterial consortia to catabolize these PAHs are discussed. Additionally, the possibility that the abiotic loss of fluorene through volatilization had a significant effect on the microbial community biodegradation of both fluorene and pyrene is examined.
本研究的目的是研究芴和芘在农业土壤中的各自生物降解和消散过程中的相互影响，并确定羟丙基-β-环糊精（HPBCD）对这些过程的影响，HPBCD 用于提高 PAHs 的生物利用度。芴的消散主要是由于非生物过程，尽管也观察到了少量的生物降解贡献。因此，HPBCD 的存在并没有增加芴的消散，也没有显著改变脱氢酶活性。与芴相反，芘的消散主要取决于生物因素，土壤中内源性微生物能够降解芘，并显著增加脱氢酶活性。HPBCD 增加了芘的生物降解速率。两种 PAHs 的共存污染并没有影响芴的演变，但显著抑制了芘的生物降解。讨论了土壤细菌群落对这些 PAHs 的不同代谢能力。此外，还研究了通过挥发从土壤中挥发损失的芴对微生物群落生物降解芴和芘的可能重大影响。
