He Tianwen, Zhao Hui, Zhao Xin, Lu Jian, Zheng Yichun, Zhang Changbin, Yin Aihua
Medical Genetics Center, Key Laboratory of Metabolic and Genetic Disease in Women and Children, Reproductive Center, Guangdong Women and Children' Hospital, Guangzhou, Guangdong 511442, China. Email:
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2016 Aug;33(4):550-4. doi: 10.3760/cma.j.issn.1003-9406.2016.04.028.
To establish an accurate, fast and simple screening method for AZF microdeletions using capillary technology and use it for clinical testing.
For each pair of primers, the 5' end of either forward or reverse primer was labeled with a FAM, JOE or TAMRA fluorescence dyes to establish multiplex quantitative fluorescence PCR systems for the establishment of a screening method of Y chromosome AZF microdeletions by capillary technology. The detection of Y chromosome AZF microdeletion was carried out on 725 cases of non-obstructive azoospermia, oligospermia or asthenospermia.
A screening method for Y chromosome AZF microdeletions using capillary technology was established. Thirty eight cases of AZF microdeletions were found among 725 cases of non-obstructive azoospermia, oligospermia or asthenospermia, which gave a deletion rate of 5.24%. Y chromosomal microdeletions were found in 8.62% of the azoospermia group, 6.75% of the oligozoospermic group, and 2.23% of the asthenospermia group.
An accurate, fast and simple screening method of Y chromosome AZF microdeletions by capillary technology has been established, which may have an important clinical value.
建立一种利用毛细管技术准确、快速且简便的AZF微缺失筛查方法，并将其应用于临床检测。
对每对引物，正向或反向引物的5′端用FAM、JOE或TAMRA荧光染料标记，建立多重定量荧光PCR系统，以确立用毛细管技术筛查Y染色体AZF微缺失的方法。对725例非梗阻性无精子症、少精子症或弱精子症患者进行Y染色体AZF微缺失检测。
建立了利用毛细管技术筛查Y染色体AZF微缺失的方法。在725例非梗阻性无精子症、少精子症或弱精子症患者中发现38例AZF微缺失，缺失率为5.24%。无精子症组Y染色体微缺失率为8.62%，少精子症组为6.75%，弱精子症组为2.23%。
已建立利用毛细管技术准确、快速且简便的Y染色体AZF微缺失筛查方法，可能具有重要临床价值。
