Fuchs Julian E, Schilling Oliver, Liedl Klaus R
Centre for Molecular Informatics, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom.
Institute of Molecular Medicine and Cell Research, University of Freiburg, Stefan-Meier- Str. 17, D-79104 Freiburg, Germany.
Curr Protein Pept Sci. 2017;18(9):905-913. doi: 10.2174/1389203717666160724211231.
Recent advances in proteomics methodologies allow for high throughput profiling of proteolytic cleavage events. The resulting substrate peptide distributions provide deep insights in the underlying macromolecular recognition events, as determinants of biomolecular specificity identified by proteomics approaches may be compared to structure-based analysis of corresponding protein-protein interfaces.
Here, we present an overview of experimental and computational methodologies and tools applied in the area and provide an outlook beyond the protein class of proteases.
We discuss here future potential, synergies and needs of the emerging overlap disciplines of proteomics and structure-based modelling.
蛋白质组学方法的最新进展使得对蛋白水解切割事件进行高通量分析成为可能。由此产生的底物肽分布为潜在的大分子识别事件提供了深入见解，因为蛋白质组学方法确定的生物分子特异性决定因素可与相应蛋白质-蛋白质界面的基于结构的分析进行比较。
在此，我们概述了该领域应用的实验和计算方法及工具，并展望了蛋白酶蛋白类别之外的情况。
我们在此讨论蛋白质组学和基于结构的建模这两个新兴交叉学科的未来潜力、协同作用及需求。
