Dias Ana M, Almeida Catarina R, Reis Celso A, Pinho Salomé S
Institute for Research and Innovation in Health, Institute of Molecular Pathology and Immunology, University of Porto (IPATIMUP), Rua Júlio Amaral de Carvalho 45, 4200-135, Porto, Portugal.
Institute of Biomedical Sciences of Abel Salazar (ICBAS), University of Porto, Porto, Portugal.
Methods Mol Biol. 2016;1389:167-76. doi: 10.1007/978-1-4939-3302-0_11.
Imaging flow cytometry is an emerging imaging technology that combines features of both conventional flow cytometry and fluorescence microscopy allowing quantification of the imaging parameters. The analysis of protein posttranslational modifications by glycosylation using imaging flow cytometry constitutes an important bioimaging tool in the glycobiology field. This technique allows quantification of the glycan fluorescence intensity, co-localization with proteins, and evaluation of the membrane/cytoplasmic expression. In this chapter we provide the guidelines to analyze glycan expression, particularly the β1,6 GlcNAc branched N-glycans, on the membrane of intestinal T cells from inflammatory bowel disease patients.
成像流式细胞术是一种新兴的成像技术，它结合了传统流式细胞术和荧光显微镜的特点，能够对成像参数进行定量分析。利用成像流式细胞术分析蛋白质糖基化修饰的翻译后修饰，是糖生物学领域一种重要的生物成像工具。该技术能够对聚糖荧光强度进行定量分析、评估其与蛋白质的共定位情况以及膜/细胞质表达情况。在本章中，我们提供了分析炎症性肠病患者肠道T细胞膜上聚糖表达的指南，特别是β1,6 N-乙酰葡糖胺分支的N-聚糖。
