使用多重实时聚合酶链反应检测博茨瓦纳常见肠胃炎病原体时植绒拭子与传统拭子的比较
A comparison of flocked swabs and traditional swabs, using multiplex real-time PCR for detection of common gastroenteritis pathogens in Botswana.
作者信息
Mokomane Margaret, Kasvosve Ishmael, Gaseitsiwe Simani, Steenhoff Andrew P, Pernica Jeffrey M, Lechiile Kwana, Luinstra Kathy, Smieja Marek, Goldfarb David M
机构信息
Department of Medical Laboratory Sciences, Faculty of Health Sciences University of Botswana, Gaborone, Botswana; Botswana National Health Laboratory, Gaborone, Botswana.
Department of Medical Laboratory Sciences, Faculty of Health Sciences University of Botswana, Gaborone, Botswana.
出版信息
Diagn Microbiol Infect Dis. 2016 Oct;86(2):141-3. doi: 10.1016/j.diagmicrobio.2016.07.007. Epub 2016 Jul 8.
Abstract
We compared the performance of flocked and matched traditional rectal swabs collected from 236 children admitted with gastroenteritis in Botswana. All samples were tested using real time multiplex-PCR assays for nine enteric pathogens. There was a 20% higher detection of Shigella from flocked swabs, but most other pathogens had similar detection rates.
摘要
我们比较了从博茨瓦纳236名患肠胃炎入院儿童采集的植绒直肠拭子和配对的传统直肠拭子的检测性能。所有样本均使用针对9种肠道病原体的实时多重聚合酶链反应检测法进行检测。植绒拭子对志贺氏菌的检测率高出20%，但大多数其他病原体的检测率相似。
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