Lim M S, Antony J J J, Islam S M Shahinul, Suhana Z, Sreeramanan S
School of Biological Sciences, Universiti Sains Malaysia, Georgetown, 11800, Penang, Malaysia.
Plant Genetic Engineering Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi, Bangladesh.
Appl Biochem Biotechnol. 2017 Jan;181(1):15-31. doi: 10.1007/s12010-016-2196-3. Epub 2016 Jul 26.
Dendrobium hybrid orchid is popular in orchid commercial industry due to its short life cycle and ability to produce various types of flower colours. This study was conducted to identify the morphological, biochemical and scanning electron microscopy (SEM) analysis in the Dendrobium sonia-28 orchid plants. In this study, 0.05 and 0.075 % of colchicine-treated Dendrobium sonia-28 (4-week-old culture) protocorm-like bodies (PLBs) were treated in different concentrations of melatonin (MEL) posttreatments (0, 0.05, 0.1, 0.5, 1, 5 and 10 μM). Morphological parameters such as number of shoots, growth index and number of PLBs were determined. In the 0.05 and 0.075 % of colchicine-treated PLBs which were posttreated with 0.05 μM MEL resulted in the highest value of the morphological parameters tested based on the number of shoots (84.5 and 96.67), growth index (16.94 and 12.15) and number of PLBs (126.5 and 162.33), respectively. SEM analysis of the 0.05 μM MEL posttreatment on both the colchicine-treated regenerated PLBs showed irregular cell lineages, and some damages occurred on the stomata. This condition might be due to the effect of plasmolyzing occurred in the cell causing irregular cell lineages.
杂交石斛兰因其生命周期短且能产生多种花色而在兰花商业产业中颇受欢迎。本研究旨在对索尼娅石斛兰28号植株进行形态学、生物化学及扫描电子显微镜（SEM）分析。在本研究中，用0.05%和0.075%秋水仙碱处理4周龄的索尼娅石斛兰28号类原球茎（PLB），然后用不同浓度的褪黑素（MEL）进行后处理（0、0.05、0.1、0.5、1、5和10μM）。测定了诸如芽数、生长指数和PLB数等形态学参数。在0.05%和0.075%秋水仙碱处理的PLB中，用0.05μM MEL进行后处理，基于芽数（84.5和96.67）、生长指数（16.94和12.15）和PLB数（126.5和162.33），分别得到了所测试形态学参数的最高值。对两种秋水仙碱处理的再生PLB用0.05μM MEL进行后处理的SEM分析显示细胞谱系不规则，气孔出现一些损伤。这种情况可能是由于细胞中发生质壁分离导致细胞谱系不规则。
