Alvarez Eduardo, Sanhueza Camila
Mycology Unit/Filamentous Fungi, Biomedical Sciences Institute, Microbiology and Mycology Program, University of Chile, Chile.
Medical Technology Department, University of Chile, Chile.
Rev Iberoam Micol. 2016 Oct-Dec;33(4):224-229. doi: 10.1016/j.riam.2016.03.007. Epub 2016 Jul 25.
Scedosporium species are considered emerging agents causing illness in immunocompromised patients. In Chile, only Scedosporium apiospermum, Scedosporium boydii and Lomentospora prolificans haven been reported previously.
The study aimed to characterize genetically Scedosporium dehoogii strains from Chilean soil samples, and assessed the antifungal susceptibility profile to classic and novel putative antifungal molecules.
In 2014, several samples were obtained during a survey of soil fungi in urban areas from Chile. Morphological and phylogenetic analyses of the internal transcribed spacer region (ITS), tubulin (TUB), and calmodulin (CAL) sequences were performed. In addition, the susceptibility profiles to classic antifungal and new putative antifungal molecules were determined.
Four strains of Scedosporium dehoogii were isolated from soil samples. The methodology confirmed the species (reported here as a new record for Chile). Antifungal susceptibility testing demonstrates the low activity of terpenes (α-pinene and geraniol) against this species. Voriconazole (VRC), posaconazole (PSC), and the hydroxyquinolines (clioquinol, and 5,7-dibromo-8-hydroxyquinoline) showed the best antifungal activity.
Our results demonstrate that Scedosporium dehoogii is present in soil samples from Chile. This study shows also that hydroxyquinolines have potential as putative antifungal molecules.
帚霉属物种被认为是引起免疫功能低下患者疾病的新兴病原体。在智利，此前仅报道过伪阿利什霉、博伊德帚霉和多育镰刀霉。
本研究旨在对来自智利土壤样本中的德霍帚霉菌株进行基因特征分析，并评估其对经典和新型潜在抗真菌分子的抗真菌药敏谱。
2014年，在智利城市地区土壤真菌调查期间获取了多个样本。对内部转录间隔区（ITS）、微管蛋白（TUB）和钙调蛋白（CAL）序列进行了形态学和系统发育分析。此外，还测定了对经典抗真菌药和新型潜在抗真菌分子的药敏谱。
从土壤样本中分离出四株德霍帚霉。该方法确定了该物种（此处报告为智利的新记录）。抗真菌药敏试验表明，萜类化合物（α-蒎烯和香叶醇）对该物种的活性较低。伏立康唑（VRC）、泊沙康唑（PSC）和羟基喹啉（氯碘羟喹和5,7-二溴-8-羟基喹啉）显示出最佳抗真菌活性。
我们的结果表明，德霍帚霉存在于智利的土壤样本中。本研究还表明，羟基喹啉作为潜在抗真菌分子具有潜力。
