土耳其多房棘球绦虫的首次分子特征分析
First Molecular Characterization of Echinococcus multilocularis in Turkey.
作者信息
Avcioglu Hamza, Guven Esin, Balkaya Ibrahim, Kirman Ridvan, Bia Mohammed Mebarek, Gulbeyen Hatice
机构信息
Department of Parasitology, Faculty of Veterinary Medicine, Atatürk University , Erzurum, Turkey .
出版信息
Vector Borne Zoonotic Dis. 2016 Sep;16(9):627-9. doi: 10.1089/vbz.2016.1983. Epub 2016 Jul 27.
This study aimed to find out the occurrence of Echinococcus multilocularis in foxes in Erzurum province, the highest endemic region for human alveolar echinococcosis in Turkey. The sedimentation and counting technique was used to reveal adult Echinococcus spp. in the intestines of foxes. One out of the 10 foxes was infected with E. multilocularis. The adult worms were analyzed morphologically and molecularly and were confirmed to be E. multilocularis by species-specific PCR. Pairwise comparisons between the 12S rRNA sequences of the E. multilocularis isolate from Erzurum and other E. multilocularis isolates showed 100% similarity of the Erzurum isolate with European isolates. With this study, the presence of E. multilocularis in a fox in Erzurum was confirmed by PCR, and molecular identification of E. multilocularis is reported for the first time in Turkey.
本研究旨在查明在土耳其人体肺泡型棘球蚴病流行程度最高的地区——埃尔祖鲁姆省的狐狸体内多房棘球绦虫的感染情况。采用沉淀计数技术来检测狐狸肠道内的棘球绦虫成虫。10只狐狸中有1只感染了多房棘球绦虫。对成虫进行了形态学和分子学分析，并通过种特异性聚合酶链反应（PCR）确认为多房棘球绦虫。将来自埃尔祖鲁姆的多房棘球绦虫分离株的12S核糖体RNA（rRNA）序列与其他多房棘球绦虫分离株进行两两比较，结果显示埃尔祖鲁姆分离株与欧洲分离株的相似性为100%。通过本研究，利用PCR证实了埃尔祖鲁姆一只狐狸体内存在多房棘球绦虫，并且在土耳其首次报道了多房棘球绦虫的分子鉴定结果。
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