Fukuta Masahiro, Masuda Yuriko, Inami Wataru, Kawata Yoshimasa
Opt Express. 2016 Jul 25;24(15):16487-95. doi: 10.1364/OE.24.016487.
We present label-free and high spatial-resolution imaging for specific cellular structures using an electron-beam excitation-assisted optical microscope (EXA microscope). Images of the actin filament and mitochondria of stained HeLa cells, obtained by fluorescence and EXA microscopy, were compared to identify cellular structures. Based on these results, we demonstrated the feasibility of identifying label-free cellular structures at a spatial resolution of 82 nm. Using numerical analysis, we calculated the imaging depth region and determined the spot size of a cathodoluminescent (CL) light source to be 83 nm at the membrane surface.
我们使用电子束激发辅助光学显微镜（EXA显微镜）对特定细胞结构进行无标记且高空间分辨率成像。通过荧光显微镜和EXA显微镜获得的染色HeLa细胞的肌动蛋白丝和线粒体图像进行比较，以识别细胞结构。基于这些结果，我们证明了在82 nm的空间分辨率下识别无标记细胞结构的可行性。通过数值分析，我们计算了成像深度区域，并确定了阴极发光（CL）光源在膜表面的光斑尺寸为83 nm。
