Fouad Amr A, Qutub Hatem O, Al-Melhim Walid N
a Department of Biomedical Sciences, Pharmacology Division, College of Medicine , King Faisal University , Al-Ahsa , Saudi Arabia.
b Department of Internal Medicine, College of Medicine , King Faisal University , Al-Ahsa , Saudi Arabia.
Toxicol Mech Methods. 2016 Sep;26(7):538-543. doi: 10.1080/15376516.2016.1211207. Epub 2016 Jul 27.
The potential nephroprotection of punicalagin (PNG) against lipopolysaccharide (LPS)-induced acute kidney injury in rats was investigated. Rats received a single i.v. dose of LPS (5 mg/kg), and treated with PNG (50 mg/kg, i.p.), 1 h before, and 1 h following LPS administration. LPS caused significant increases of serum creatinine and neutrophil gelatinase-associated lipocalin. LPS also resulted in significant increases in interleukin-18, tumor necrosis factor-α, interleukin-6, malondialdehyde, nitric oxide, Bax/Bcl-2 ratio and myeloperoxidase, inducible nitric oxide synthase, caspases 3, 8 and 9 activities, and a significant decrease in total antioxidant capacity in kidney tissues. PNG significantly ameliorated the alterations in the measured parameters. Additionally, PNG attenuated the histopathological injury and reduced kidney injury molecule-1 expression in kidneys of rats that received LPS. It was concluded that PNG ameliorated endotoxemic acute kidney injury in rats by counteracting inflammation, oxidative/nitrative stress and apoptosis.
研究了石榴皮苷（PNG）对脂多糖（LPS）诱导的大鼠急性肾损伤的潜在肾保护作用。大鼠静脉注射单次剂量的LPS（5mg/kg），并在LPS给药前1小时和给药后1小时腹腔注射PNG（50mg/kg）。LPS导致血清肌酐和中性粒细胞明胶酶相关脂质运载蛋白显著增加。LPS还导致肾组织中白细胞介素-18、肿瘤坏死因子-α、白细胞介素-6、丙二醛、一氧化氮、Bax/Bcl-2比值和髓过氧化物酶、诱导型一氧化氮合酶、半胱天冬酶3、8和9活性显著增加，总抗氧化能力显著降低。PNG显著改善了所测参数的变化。此外，PNG减轻了接受LPS的大鼠肾脏的组织病理学损伤并降低了肾损伤分子-1的表达。得出的结论是，PNG通过对抗炎症、氧化/硝化应激和细胞凋亡改善了大鼠内毒素血症性急性肾损伤。
