Shinya Minori
Department of Biology, Keio University, 4-1-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa, 223-8521, Japan.
Methods Mol Biol. 2016;1451:107-18. doi: 10.1007/978-1-4939-3771-4_8.
Genetically homogeneous populations such as inbred strains are valuable experimental tools in various fields of biomedical analyses. In many animals, inbred strains are established by consecutive sib-pair mating for a minimum of 20 generations. As the generation proceeds, fitness of the population reduces usually. Therefore, in order to establish inbred strains, the important point is the selection of pairs in good condition at each generation. Here, I describe the procedure and tips for generating inbred strains in zebrafish.
基因同质群体，如近交系，是生物医学分析各个领域中很有价值的实验工具。在许多动物中，近交系是通过连续的同胞配对交配至少20代建立起来的。随着代数的增加，群体的适应性通常会降低。因此，为了建立近交系，关键在于每一代选择状态良好的配对。在此，我描述在斑马鱼中产生近交系的步骤和技巧。
