Wen Min, Fu Xueyuan, Han Xiuqing, Hu Xiaoqian, Dong Ping, Xu Jie, Xue Yong, Wang Jingfeng, Xue Changhu, Wang Yuming
College of Food Science and Engineering, Ocean University of China.
J Nutr Sci Vitaminol (Tokyo). 2016;62(3):170-7. doi: 10.3177/jnsv.62.170.
Circadian rhythms control aspects of physiological events, including lipid metabolism, showing rhythmic fluctuation over 24 h. Therefore, it is not sufficient to evaluate thoroughly how dietary components regulate lipid metabolism with a single time-point assay. In the present study, a time-course study was performed to analyze the effect of sea cucumber saponin echinoside A (EA) on lipid metabolism over 24 h. Results showed that EA lowered the levels of TC and TG in both serum and liver at most time-points during the 24 h. Activities of hepatic lipogenic enzymes and lipolytic enzymes were inhibited and elevated respectively by EA to varied degrees at different time-points. Meanwhile, parallel variation trends of gene expression involved in fatty acid synthesis and β-oxidation were observed accordingly. The interaction between EA and lipid metabolism showed a time-dependent effect. Overall, EA impaired fatty acid synthesis and enhanced mitochondrial fatty acid β-oxidation in ad libitum feeding over 24 h.
昼夜节律控制着包括脂质代谢在内的生理活动的各个方面，脂质代谢在24小时内呈现出节律性波动。因此，仅通过单一时间点检测来全面评估饮食成分如何调节脂质代谢是不够的。在本研究中，进行了一项时间进程研究，以分析海参皂苷棘皮苷A（EA）在24小时内对脂质代谢的影响。结果表明，在24小时内的大多数时间点，EA均降低了血清和肝脏中总胆固醇（TC）和甘油三酯（TG）的水平。在不同时间点，EA对肝脏生脂酶和脂解酶的活性分别有不同程度的抑制和升高作用。同时，相应地观察到脂肪酸合成和β-氧化相关基因表达的平行变化趋势。EA与脂质代谢之间的相互作用呈现出时间依赖性效应。总体而言，在24小时自由采食期间，EA损害脂肪酸合成并增强线粒体脂肪酸β-氧化。
