Wang Fang, Zhang Xueliang, Wang Yan, Sun Zhaohui
Department of Clinical Laboratory, The Second Hospital, Shandong University, Jinan, Shandong, China.
Jinan Blood Center, Jinan, Shandong, China.
Ann Clin Lab Sci. 2016 Jul;46(4):374-9.
HBV is the most common and serious infectious disease in China. Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) belongs to the TNF-super family and can induce apoptosis in addition to other biological effects. It has been found that the occurrence of chronic hepatitis B is related to polymorphisms of many apoptotic factors. This study therefore aims to investigate the association of TRAIL polymorphism with chronic hepatitis B by observing the polymorphism and gene frequency of the TRAIL gene at 1525G/A and 1595C/T of the 3'-UTR among people of the Chinese Han ethnicity.
This study followed a case-control design. Polymorphisms of the TRAIL gene were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods in 187 patients with chronic hepatitis B and 142 healthy controls.
The alleles of TRAIL at 1525G/A and 1595C/T have achieved genetic equilibrium in normal individuals (P<0.05) and patients with chronic hepatitis B(P=0.547). The genotype frequencies of TRAIL at 1525GG/1595CC in female patients were significantly higher than those in male patients. The frequencies of the AA genotype at 1525G/A and the TT genotype at 1595C/T sites were significantly lower in patients with chronic hepatitis B with HBeAg positive than in healthy controls (10.5 vs 23.2%, P<0.05). The frequency of the AA genotype at 1525G/A and TT genotype at 1595C/T sites were significantly lower in the chronic hepatitis B individuals than in normal controls (12.3 vs 23.2%, P < 0.05).
TRAIL may be related to susceptibility to hepatitis B.
乙肝病毒是中国最常见且最严重的传染病。肿瘤坏死因子（TNF）相关凋亡诱导配体（TRAIL）属于TNF超家族，除具有其他生物学效应外，还可诱导细胞凋亡。已发现慢性乙型肝炎的发生与多种凋亡因子的多态性有关。因此，本研究旨在通过观察中国汉族人群中TRAIL基因3'-UTR区1525G/A和1595C/T位点的多态性及基因频率，探讨TRAIL多态性与慢性乙型肝炎的相关性。
本研究采用病例对照设计。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，对187例慢性乙型肝炎患者和142例健康对照者的TRAIL基因多态性进行分析。
TRAIL基因1525G/A和1595C/T位点的等位基因在正常个体（P<0.05）和慢性乙型肝炎患者中（P=0.547）均达到遗传平衡。女性患者中TRAIL基因1525GG/1595CC基因型频率显著高于男性患者。HBeAg阳性的慢性乙型肝炎患者中，1525G/A位点AA基因型频率和1595C/T位点TT基因型频率显著低于健康对照者（10.5%对23.2%，P<0.05）。慢性乙型肝炎患者中1525G/A位点AA基因型频率和1595C/T位点TT基因型频率显著低于正常对照者（12.3%对23.2%，P<0.05）。
TRAIL可能与乙型肝炎易感性有关。
