Fu Yung-Chieh, Liao I-Chuang, Chen Hung-Mo, Yan Jing-Jou
Department of Pathology, National Cheng Kung University Hospital, Taiwan.
Department of Pathology, National Cheng Kung University Hospital, Taiwan Department of Pathology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
Ann Clin Lab Sci. 2016 Jul;46(4):412-7.
The Abbott RealTime MTB assay, launched in June 2014, has been shown to have a competitive performance in the detection of the Mycobacterium tuberculosis (MTB) complex in respiratory specimens. The present study was conducted to investigate the usefulness of the Abbott MTB Realtime assay in the detection of MTB in formalin-fixed paraffin-embedded (FFPE) tissues.
A total of 96 FFPE specimens obtained from microbiologically proven MTB cases (N=60) and nontuberculous Mycobacterium cases (N=36) were analyzed. The performance of the Abbott MTB Realtime assay was compared with that of the Roche Cobas TaqMan MTB assay.
The overall sensitivity and specificity of the Abbott assay were 63.3% and 97.2%, respectively, compared with 11.7% and 100% for the Cobas assay. The detection rate of the Abbott assay was much higher among 37 acid-fast-positive specimens than among 23 acid-fast-negative specimens (89.3% versus 21.7%, respectively). The detection rate of the assay was higher among 29 resection specimens than among 31 small biopsy specimens (86.2% versus 41.9%, respectively).
Our results suggest that the Abbott RealTime MTB assay can be used to differentiate MTB from nontuberculous mycobacterial infections in acid-fast-positive FFPE tissues.
2014年6月推出的雅培实时结核分枝杆菌检测法已被证明在检测呼吸道标本中的结核分枝杆菌（MTB）复合体方面具有竞争力。本研究旨在探讨雅培MTB实时检测法在福尔马林固定石蜡包埋（FFPE）组织中检测MTB的实用性。
对96份从微生物学确诊的MTB病例（N = 60）和非结核分枝杆菌病例（N = 36）获得的FFPE标本进行分析。将雅培MTB实时检测法的性能与罗氏Cobas TaqMan MTB检测法进行比较。
雅培检测法的总体敏感性和特异性分别为63.3%和97.2%，而Cobas检测法分别为11.7%和100%。在37份抗酸阳性标本中，雅培检测法的检出率远高于23份抗酸阴性标本（分别为89.3%和21.7%）。该检测法在29份切除标本中的检出率高于31份小活检标本（分别为86.2%和41.9%）。
我们的结果表明，雅培实时MTB检测法可用于在抗酸阳性的FFPE组织中将MTB与非结核分枝杆菌感染区分开来。
