Young H E, Carrino D A, Caplan A I
Department of Biology, Case Western Reserve University, Cleveland, Ohio 44106.
J Morphol. 1989 Jul;201(1):85-103. doi: 10.1002/jmor.1052010108.
The leg musculature from 11, 14, and 17 day chick embryos was analyzed histochemically to investigate the temporal and spatial distribution of various types of sulfated glycosaminoglycans present during skeletal muscle development. Types of glycans were identified by selective degradation with specific glycosidases and nitrous acid coupled with Alcian blue staining procedures for sulfated polyanions and with [35S]sulfate autoradiography. On day 11, radiolabeled chondroitin sulfate glycosaminoglycans are localized extracellularly in both the myogenic and connective tissue cell populations. By day 17, incorporation of [35S]sulfate into chondroitin sulfate is substantially reduced, although Alcian blue-stained chondroitin sulfate molecules are still detectable. With increasing age and developmental state of the tissues, radiolabeled and stained dermatan sulfate and heparan sulfate progressively increase in relative quantity compared to chondroitin sulfate both in muscle and in associated connective tissue elements. These changes in glycosaminoglycans correlate well with similar changes previously determined biochemically and further document the alterations in extracellular matrix components during embryonic skeletal myogenesis.
对11日龄、14日龄和17日龄鸡胚的腿部肌肉组织进行了组织化学分析，以研究骨骼肌发育过程中存在的各种硫酸化糖胺聚糖的时间和空间分布。通过用特定糖苷酶和亚硝酸进行选择性降解，结合用于硫酸化聚阴离子的阿尔辛蓝染色程序以及[35S]硫酸盐放射自显影来鉴定聚糖类型。在第11天，放射性标记的硫酸软骨素糖胺聚糖定位于成肌细胞和结缔组织细胞群体的细胞外。到第17天，尽管仍可检测到阿尔辛蓝染色的硫酸软骨素分子，但[35S]硫酸盐掺入硫酸软骨素的量大幅减少。随着组织年龄和发育状态的增加，与硫酸软骨素相比，放射性标记和染色的硫酸皮肤素和硫酸乙酰肝素在肌肉和相关结缔组织成分中的相对量逐渐增加。糖胺聚糖的这些变化与先前通过生化方法确定的类似变化密切相关，并进一步证明了胚胎骨骼肌生成过程中细胞外基质成分的改变。
