在人类心肌中未检测到黄嘌呤氧化酶。

Absence of detectable xanthine oxidase in human myocardium.

作者信息

Grum C M, Gallagher K P, Kirsh M M, Shlafer M

机构信息

Department of Internal Medicine, University of Michigan Medical School, Ann Arbor.

出版信息

J Mol Cell Cardiol. 1989 Mar;21(3):263-7. doi: 10.1016/0022-2828(89)90741-4.

DOI:10.1016/0022-2828(89)90741-4

PMID:2746653

Abstract

The enzyme xanthine oxidase has been implicated as a generator of toxic oxygen metabolites that contribute to ischemic injury. Because substantial species variability has been demonstrated and because there are minimal human data available, the relevance of xanthine oxidase to human heart damage has been in doubt. We report the absence of xanthine oxidase activity in nine human heart biopsy specimens obtained during cardiac surgery, and in two larger samples obtained during heart transplantation. A sensitive radiochemical assay was used to assess enzyme activity. Our findings imply that oxygen free radicals generated by xanthine oxidase are not relevant in terms of human myocardial injury.

摘要

黄嘌呤氧化酶被认为是有毒氧代谢产物的生成源，这些代谢产物会导致缺血性损伤。由于已证实存在显著的物种差异，且可用的人体数据极少，黄嘌呤氧化酶与人类心脏损伤的相关性一直存疑。我们报告了在心脏手术期间获取的9份人类心脏活检标本以及心脏移植期间获取的2份更大样本中均不存在黄嘌呤氧化酶活性。使用一种灵敏的放射化学分析法来评估酶活性。我们的研究结果表明，黄嘌呤氧化酶产生的氧自由基与人类心肌损伤无关。