Health & Environment Department, AIT Austrian Institute of Technology , Vienna, Austria.
Institute of Materials Chemistry, Vienna University of Technology , Vienna, Austria.
ACS Appl Mater Interfaces. 2016 Aug 24;8(33):21228-35. doi: 10.1021/acsami.6b06927. Epub 2016 Aug 10.
A better understanding of the electrodynamic behavior of cells interacting with electric fields would allow for novel scientific insights and would lead to the next generation of cell manipulation, diagnostics, and treatment. Here, we introduce a promising electrode design by using metal oxide high-k dielectric passivation. The thermally generated dielectric passivation layer enables efficient electric field coupling to the fluid sample comprising cells while simultaneously decoupling the electrode ohmically from the electrolyte, allowing for better control and adjustability of electric field effects due to reduced electrochemical reactions at the electrode surface. This approach demonstrates cell-size specific lysis with electric fields in a microfluidic flow-through design resulting in 99.8% blood cell lysis at 6 s exposure without affecting the viability of Gram-positive and Gram-negative bacterial spike-ins. The advantages of this new approach can support next-generation investigations of electrodynamics in biological systems and their exploitation for cell manipulation in multiple fields of medicine, life science, and industry.
更好地了解细胞与电场相互作用的电动行为将为新的科学见解提供可能，并将引领下一代细胞操作、诊断和治疗。在这里，我们通过使用金属氧化物高介电常数电介质钝化来引入一种很有前途的电极设计。热生成的介电钝化层能够有效地将电场耦合到包含细胞的流体样品中，同时使电极与电解质在欧姆上解耦，从而由于电极表面的电化学反应减少而更好地控制和调节电场效应。这种方法在微流控直通设计中展示了针对细胞大小的特异性裂解，在 6 秒暴露下，99.8%的血细胞裂解，而不会影响革兰氏阳性和革兰氏阴性细菌 Spike-ins 的活力。这种新方法的优势可以为生物系统电动学的下一代研究提供支持，并将其应用于医学、生命科学和工业的多个领域的细胞操作。
