Romè Chiara, Huang Xin-Yuan, Danku John, Salt David E, Sebastiani Luca
BioLabs, Institute of Life Sciences, Scuola Superiore Sant'Anna, Piazza Martiri della Libertà 33, I-56127 Pisa, Italy.
Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 3UU, United Kingdom.
J Plant Physiol. 2016 Sep 1;202:83-91. doi: 10.1016/j.jplph.2016.07.009. Epub 2016 Jul 16.
Cadmium (Cd) is a heavy metal toxic to humans and its occurrence in soils represents a significant environmental problem. Poplar trees may provide one possible option to help remove Cd contamination from soil. However, before this is practicable, the ability of poplar to accumulate Cd needs to be enhanced. A better understanding of the genes involved in Cd accumulation in poplar would help to achieve this goal. Here, we monitored the expression of genes known to be involved in Cd uptake, accumulation and translocation from other species, in order to provide information on their potential role in Cd accumulation in poplar. Cd concentration in poplar was significantly higher in roots than in stem and leaves in Cd treated plants. Expression of the poplar homologues of IRT1, NRAMP and OPT3 was initially increased after exposure to Cd but reduced after longer term Cd exposure. Exposure to Cd also influenced the accumulation of Fe, Ca, Cu, Mg and Mn in poplar. In particular, Cd treated plants had a higher concentration of Fe, Ca, Cu, and Mg in leaves and stem compared to control plants after one day and one week of experiment; while in roots after one month Cd treated plants had a lower concentration of Mn, Fe, Cu, Co, and Mg.
镉（Cd）是一种对人类有毒的重金属，其在土壤中的存在是一个重大的环境问题。杨树可能是帮助去除土壤中镉污染的一种可行选择。然而，在这可行之前，需要提高杨树积累镉的能力。更好地了解杨树中参与镉积累的基因将有助于实现这一目标。在此，我们监测了已知参与其他物种镉吸收、积累和转运的基因的表达，以便提供它们在杨树镉积累中潜在作用的信息。在镉处理的植株中，杨树根部的镉浓度显著高于茎和叶。IRT1、NRAMP和OPT3杨树同源基因的表达在接触镉后最初增加，但在长期接触镉后降低。接触镉也影响了杨树中铁、钙、铜、镁和锰的积累。特别是，在实验一天和一周后，与对照植株相比，镉处理的植株叶片和茎中的铁、钙、铜和镁浓度更高；而在一个月后，镉处理的植株根部的锰、铁、铜、钴和镁浓度更低。
