Sharma Arjun, Enderlein Jörg, Kumbhakar Manoj
Radiation & Photochemistry Division, Bhabha Atomic Research Center , Mumbai 400085, India.
III Institute of Physics, Georg August University , 37077 Göttingen, Germany.
J Phys Chem Lett. 2016 Aug 18;7(16):3137-41. doi: 10.1021/acs.jpclett.6b01467. Epub 2016 Aug 2.
We present a novel fluorescence spectroscopic method, which combines fluorescence antibunching, time-correlated single-photon counting (TCSPC), and steady-state emission spectroscopy, to study chemical reactions at the single molecule level. We exemplify our method on investigating intermolecular fluorescence quenching of Rhodamine110 by aniline. We demonstrate that the combination of measurements of fluorescence antibunching, fluorescence lifetime, and fluorescence steady state intensity, captures the full picture of the complex quenching kinetics, which involves static and dynamics quenching, and which cannot be seen by steady-state or lifetime measurements alone.
我们提出了一种新颖的荧光光谱方法，该方法结合了荧光反聚束、时间相关单光子计数（TCSPC）和稳态发射光谱，用于在单分子水平上研究化学反应。我们以研究苯胺对罗丹明110的分子间荧光猝灭为例来说明我们的方法。我们证明，荧光反聚束测量、荧光寿命测量和荧光稳态强度测量的结合，能够全面呈现复杂的猝灭动力学过程，其中涉及静态猝灭和动态猝灭，而仅通过稳态或寿命测量是无法观察到这些的。
