Ryan S J, Gavard E J, Cheeseman A E, Cohen J B, Whipps C M
J Zoo Wildl Med. 2016 Jun;47(2):659-62. doi: 10.1638/2015-0157.1.
From June 2014 to June 2015, capillary tube collections of blood were obtained concurrently with ear clips of trapped free-ranging, globally vulnerable New England cottontails (NEC; Sylvilagus transitionalis) and eastern cottontail rabbits (EC; Sylvilagus floridanus) in the Hudson Valley region of New York, United States. Species identification (NEC, EC) and sex (NEC) were determined genetically using a mitochondrial DNA assay and Y chromosome marker, respectively. Hematocrit values were obtained using a microhematocrit centrifuge. We provide the reference values 35.15-49.55 (2.5 and 97.5 percentiles) and 90% confidence intervals (CI) [lower: 33.00, 36.08; upper: 46.95, 51.00], for hematocrit of NEC. The mean hematocrit for NEC was 42.35% (SE = 0.58, n = 47) and a comparative contemporaneous mean in the same area for EC [39.96 (SE = 0.81, n = 26)], which was significantly different from NEC (P = 0.02). There was a significant sex difference for NEC [male: 43.99 (SE = 1.02, n = 28); female: 39.92 (SE = 0.78, n = 19), P < 0.0001], though not for EC.
2014年6月至2015年6月期间，在美国纽约哈德逊河谷地区，从诱捕的全球易危物种新英格兰棉尾兔（NEC；过渡棉兔，学名Sylvilagus transitionalis）和东部棉尾兔（EC；佛罗里达棉兔，学名Sylvilagus floridanus）身上采集耳夹样本的同时，也采集了毛细管血液样本。分别使用线粒体DNA检测和Y染色体标记，通过基因手段确定物种（NEC、EC）和性别（NEC）。使用微量血细胞比容离心机获得血细胞比容值。我们给出了新英格兰棉尾兔血细胞比容的参考值35.15 - 49.55（第2.5和第97.5百分位数）以及90%置信区间（CI）[下限：33.00，36.08；上限：46.95，51.00]。新英格兰棉尾兔的平均血细胞比容为42.35%（标准误 = 0.58，n = 47），同一地区东部棉尾兔同期的比较平均值为[39.96（标准误 = 0.81，n = 26）]，与新英格兰棉尾兔有显著差异（P = 0.02）。新英格兰棉尾兔存在显著的性别差异[雄性：43.99（标准误 = 1.02，n = 28）；雌性：39.92（标准误 = 0.78，n = 19），P < 0.0001]，而东部棉尾兔则不存在。
