Nakamaki T, Sakashita A, Sano M, Hino K, Suzuki K, Tomoyasu S, Tsuruoka N
Rinsho Ketsueki. 1989 Feb;30(2):149-57.
The effects of glucocorticoid on the differentiation of myeloid leukemia cells were examined. Dexamethasone at 10(-6)M or 10(-7)M revealed marked effects not only on leukemic blasts' survival but also on its' differentiation in vitro. In 10 of 17 cases of myeloid leukemia, the obvious morphological and functional differentiation induction effects were observed in vitro. The direction of differentiation were differed in leukemia cell lineage and in cases. Granulocytic differentiation in M2 cells, mono-macrophage differentiation in M5 cells and either granulocytic or monocytic differentiation in M4 cells were induced. A AML (M2), it's leukemia cells were induced into granulocytic pathway by dexamethasone in vitro, was treated with prednisolone (40 mg per day).Ara-C (15 mg per day). The increase in peripheral leukocyte count and the decrease in immature cells were observed simultaneously. The peripheral leukocytes mainly consisted of intermediate forms of granulocytes and Pelger-Huët like neutrophils probably originated from leukemia cells. After that course, abnormal clone was eliminated from bone marrow. A AMoL, it's leukemia cells were induced into macrophage like cells completely by dexamethasone in vitro, was treated with prednisolone (30 mg per day) and complete remission was obtained without passing through a hematological nadir. It is indicated that anti-tumor effects of glucocorticoid on myeloid leukemia cells are closely related to it's differentiation inducing effect and glucocorticoid can be used as the drug intending for the differentiation induction therapy of acute myeloid leukemia.
研究了糖皮质激素对髓系白血病细胞分化的影响。10(-6)M或10(-7)M的地塞米松不仅对白血病原始细胞的存活有显著影响，而且对其体外分化也有显著影响。在17例髓系白血病患者中的10例中，体外观察到明显的形态学和功能分化诱导作用。白血病细胞系别和病例之间的分化方向不同。诱导M2细胞向粒细胞分化、M5细胞向单核-巨噬细胞分化以及M4细胞向粒细胞或单核细胞分化。1例急性髓系白血病（M2），其白血病细胞在体外被地塞米松诱导进入粒细胞途径，接受泼尼松龙（每天40mg）和阿糖胞苷（每天15mg）治疗。同时观察到外周血白细胞计数增加和未成熟细胞减少。外周血白细胞主要由粒细胞的中间形式和可能起源于白血病细胞的Pelger-Huët样中性粒细胞组成。经过该疗程后，骨髓中异常克隆被清除。1例急性单核细胞白血病，其白血病细胞在体外被地塞米松完全诱导为巨噬样细胞，接受泼尼松龙（每天30mg）治疗，未经历血液学最低点就获得了完全缓解。表明糖皮质激素对髓系白血病细胞的抗肿瘤作用与其分化诱导作用密切相关，糖皮质激素可作为急性髓系白血病分化诱导治疗的药物。
