de Azevedo Maria Isabel, Ferreiro Laerte, Da Silva Aleksandro S, Tonin Alexandre A, Monteiro Danieli Urach, Casali Emerson A, Moritz Cesar E J, Schirmbeck Gabriel H, Cardoso Valesca V, Flores Mariana M, Fighera Rafael, Stefani Lenita M, Santurio Janio M
Laboratório de Micologia, Departamento de Patologia e Clinica Animal, Faculdade de Veterinária (UFRGS), Porto Alegre, RS, Brazil; Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Laboratório de Micologia, Departamento de Patologia e Clinica Animal, Faculdade de Veterinária (UFRGS), Porto Alegre, RS, Brazil.
Microb Pathog. 2016 Oct;99:36-40. doi: 10.1016/j.micpath.2016.07.015. Epub 2016 Jul 25.
The present study was carried out to assess the participation of purines in the activation and modulation of inflammatory response of rats experimentally infected by Cryptococcus neoformans. Twenty four Wistar rats were divided into two groups of 12 animals each: Group A - uninfected control group and Group B - infected by C. neoformans. Blood was collected 20 and 50 days post-infection (PI) from six animals of each group in order to verify purine levels (adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), adenosine (ADO), inosine (INO), hypoxanthine (HYPO), xanthine (XAN) and uric acid (URIC)). ATP levels were significantly increased (P < 0.05) in serum from infected animals on days 20 and 50 PI, as well as adenosine levels after 20 days PI on rats. On day 50 PI, levels of adenosine and uric acid were also reduced, but the levels of inosine and xanthine increased in animals infected by the fungus (P < 0.05). Therefore, it was possible to conclude that the purine levels in serum were altered and that these changes may be able to influence the pathogenesis of the disease caused by C. neoformans due the participation of purines (ATP and adenosine main) in the activation and modulation of inflammatory response.
本研究旨在评估嘌呤在实验性感染新型隐球菌的大鼠炎症反应激活和调节中的作用。24只Wistar大鼠分为两组，每组12只：A组为未感染对照组，B组为新型隐球菌感染组。在感染后20天和50天，从每组6只动物采集血液，以检测嘌呤水平（三磷酸腺苷（ATP）、二磷酸腺苷（ADP）、一磷酸腺苷（AMP）、腺苷（ADO）、肌苷（INO）、次黄嘌呤（HYPO）、黄嘌呤（XAN）和尿酸（URIC））。在感染后20天和50天，感染动物血清中的ATP水平显著升高（P<0.05），感染后20天大鼠的腺苷水平也显著升高。在感染后50天，感染真菌的动物腺苷和尿酸水平也降低，但肌苷和黄嘌呤水平升高（P<0.05）。因此，可以得出结论，血清中的嘌呤水平发生了改变，并且由于嘌呤（主要是ATP和腺苷）参与炎症反应的激活和调节，这些变化可能会影响新型隐球菌引起的疾病的发病机制。
