Hong Soomin, Jo Junghyun, Kim Hyung Joon, Lee Jeoung Eun, Shin Dong Hyuk, Lee Sung-Geum, Baek Ahmi, Shim Sung Han, Lee Dong Ryul
1 Department of Biomedical Science, College of Life Science, CHA University , Seongnam, Korea.
2 Fertility Center, CHA Gangnam Medical Center, College of Medicine, CHA University , Seoul, Korea.
Stem Cells Dev. 2016 Sep 15;25(18):1376-85. doi: 10.1089/scd.2016.0076. Epub 2016 Aug 30.
Although many factors have been identified to be involved in the development of the neuroectoderm during embryogenesis, it is still important to identify novel factors that convert undifferentiated embryonic cells into neuroectoderm. RuvB-like protein 2 (Ruvbl2) is known to regulate gene expression via chromatin remodeling by participating in multi-protein complexes, but its role during embryonic development is not well known. In this study, we established Ruvbl2-overexpressing mouse embryonic stem cells and examined their capacity to specifically differentiate into neuroectoderm and confirmed the specific expression of RUVBL2 in early embryonic neuroectoderm. Our results suggest that Ruvbl2 has a role in the differentiation of neuroectoderm during early embryogenesis.
尽管在胚胎发生过程中已确定许多因素参与神经外胚层的发育，但识别将未分化胚胎细胞转化为神经外胚层的新因素仍然很重要。已知RuvB样蛋白2（Ruvbl2）通过参与多蛋白复合物进行染色质重塑来调节基因表达，但其在胚胎发育过程中的作用尚不清楚。在本研究中，我们建立了过表达Ruvbl2的小鼠胚胎干细胞，并检测了它们特异性分化为神经外胚层的能力，证实了RUVBL2在早期胚胎神经外胚层中的特异性表达。我们的结果表明，Ruvbl2在胚胎早期发育过程中对神经外胚层的分化起作用。
