Xia Weiguang, Fouad Ahmed Mohamed, Chen Wei, Ruan Dong, Wang Shuang, Fan Qiuli, Wang Ying, Cui Yiyan, Zheng Chuntian
Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture, State Key Laboratory of Livestock and Poultry Breeding, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, P. R. China.
Department of Animal Production, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
Poult Sci. 2017 Jan 1;96(1):144-150. doi: 10.3382/ps/pew205. Epub 2016 Jul 28.
This study aimed to establish the arginine requirements of Longyan ducks from 17 to 31 wk of age based on egg production, egg quality, plasma, and ovarian indices, as well as the expression of vitellogenesis-related genes. In total, 660 Longyan ducks with similar body weight at 15 wk of age were assigned randomly to 5 treatments, each with 6 replicates of 22 birds, and fed a corn-corn gluten meal basal diet (0.66% arginine) supplemented with either 0, 0.20%, 0.40%, 0.60%, or 0.80% arginine. Dietary arginine did not affect egg production by laying ducks, but it increased (linear, P < 0.01) the egg weight at 22 to 31 and 17 to 31 wk of age. Dietary arginine increased the yolk color score (linearly, P < 0.05) and the yolk percentage (quadratic, P < 0.05), where the maximum values were obtained with 1.26% arginine. Dietary arginine affected the total shell percentage and shell thickness, with the highest values using 1.46% arginine (P < 0.01). The weight and number of small yellow follicles (SYFs) increased (quadratic, P < 0.05) with the dietary arginine level and there was a quadratic response (P < 0.05) in terms of the SYFs weight/ovarian weight; the highest values were obtained in ducks fed 1.26% arginine. The plasma arginine concentration exhibited a quadratic (P < 0.05) response to dietary arginine. The plasma progesterone concentration decreased (linear, P < 0.05) as dietary arginine increased. The mRNA abundance of the very low density lipoprotein receptor-b increased in the second large yellow follicle membranes (quadratic, P < 0.05) with the dietary arginine level, where the highest value occurred with 1.26% arginine. According to the regression model, the dietary arginine requirements for Longyan laying ducks aged 17 to 31 wk are 1.06%, 1.13%, 1.22%, and 1.11% to obtain the maximum yolk percentage, SYFs number, SYFs weight, and SYFs weight/ovarian weight, respectively.
本研究旨在基于产蛋性能、蛋品质、血浆和卵巢指标以及卵黄生成相关基因的表达，确定17至31周龄龙岩鸭的精氨酸需求量。总共660只15周龄体重相近的龙岩鸭被随机分为5组处理，每组6个重复，每个重复22只，分别饲喂补充0、0.20%、0.40%、0.60%或0.80%精氨酸的玉米-玉米蛋白粉基础日粮（精氨酸含量0.66%）。日粮精氨酸对产蛋鸭的产蛋性能没有影响，但增加了（线性，P<0.01）22至31周龄以及17至31周龄时的蛋重。日粮精氨酸增加了蛋黄颜色评分（线性，P<0.05）和蛋黄比例（二次曲线，P<0.05），精氨酸含量为1.26%时达到最大值。日粮精氨酸影响了蛋壳总比例和蛋壳厚度，精氨酸含量为1.46%时最高（P<0.01）。小黄卵泡（SYF）的重量和数量随着日粮精氨酸水平的增加而增加（二次曲线，P<0.05），SYF重量/卵巢重量方面存在二次曲线响应（P<0.05）；精氨酸含量为1.26%的日粮组鸭达到最高值。血浆精氨酸浓度对日粮精氨酸呈二次曲线响应（P<0.05）。随着日粮精氨酸增加，血浆孕酮浓度降低（线性，P<0.05）。第二大黄色卵泡膜中极低密度脂蛋白受体-b的mRNA丰度随着日粮精氨酸水平的增加而增加（二次曲线，P<0.05），精氨酸含量为1.26%时达到最高值。根据回归模型，17至31周龄龙岩产蛋鸭获得最大蛋黄比例、SYF数量、SYF重量和SYF重量/卵巢重量时，日粮精氨酸需求量分别为1.06%、1.13%、1.22%和1.11%。
