College of Animal Sciences, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, P.R. China.
College of Animal Sciences, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, P.R. China.
Poult Sci. 2020 Jan;99(1):578-589. doi: 10.3382/ps/pez545. Epub 2019 Dec 30.
N-carbamylglutamate (NCG), an analogue of N-acetyl-L-glutamate (NAG), can increase arginine synthesis in mammals and improve the reproductive performance. However, the effect of NCG on poultry laying performance is still unclear. This study investigated the effect of dietary NCG on development of chicken ovarian follicles. The dosage and timing for NCG administration were evaluated for its effect on follicular development. Results showed that supplementation with 1% NCG in the diet for 14 D led to accelerated development of growing follicles (over 60 μm in oocyte diameter) and significantly increased feed intake and feed efficiency. Plasma amino acids (AA) analysis showed that feeding with 1% NCG significantly increased of plasma AA levels. RNA-seq analysis revealed that NCG supplementation upregulated expression of genes related to angiogenesis and cell proliferation, but downregulated expression of apoptosis-related genes. Meanwhile, RT-qPCR and Western blot analysis validated the RNA-seq results. Moreover, NCG enhanced plasma NO level; upregulated expression of PKG-I, Raf1, and p-p38; and increased angiogenesis of the ovaries. In conclusion, dietary NCG (1% for 14 D) can promote development of ovarian follicles by increasing angiogenesis in ovaries of the chicken.
N- 氨甲酰谷氨酸（NCG）是 N- 乙酰-L-谷氨酸（NAG）的类似物，可增加哺乳动物的精氨酸合成并改善繁殖性能。然而，NCG 对家禽产蛋性能的影响尚不清楚。本研究探讨了日粮 NCG 对鸡卵巢卵泡发育的影响。评估了 NCG 给药的剂量和时间对卵泡发育的影响。结果表明，日粮中添加 1%的 NCG 持续 14 天可加速生长卵泡（卵母细胞直径超过 60μm）的发育，并显著增加采食量和饲料效率。血浆氨基酸（AA）分析表明，添加 1%的 NCG 可显著提高血浆 AA 水平。RNA-seq 分析显示，NCG 补充可上调与血管生成和细胞增殖相关的基因表达，但下调与凋亡相关的基因表达。同时，RT-qPCR 和 Western blot 分析验证了 RNA-seq 的结果。此外，NCG 可增强血浆中 NO 水平，上调 PKG-I、Raf1 和 p-p38 的表达，并增加卵巢的血管生成。综上所述，日粮 NCG（14 天，1%）可通过增加鸡卵巢的血管生成来促进卵泡的发育。
