在肝细胞溶胶中，配体蛋白保留胆红素结合能力，而白蛋白丧失胆红素结合能力。
Ligandin retains and albumin loses bilirubin binding capacity in liver cytosol.
作者信息
Listowsky I, Gatmaitan Z, Arias I M
出版信息
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1213-6. doi: 10.1073/pnas.75.3.1213.
Abstract
Circular dichroism methods were used to detect bilirubin-ligandin interactions in rat liver cytosol and fractions obtained at various stages during purification of ligandin. Ligandin retained its capacity to bind bilirubin in the presence of components of liver supernatant, but albumin, which binds bilirubin in serum, lost the capacity to bind bilirubin in liver supernatant. This was attributed to a greater binding specificity exhibited by ligandin. In their respective physiological milieus, albumin and ligandin are structurally adapted to bind ligands: albumin in serum, and ligandin in the cytosol of the liver cell. These studies are consistent with the hypothesis that the concentration of ligandin within the liver could regulate the net flux of certain organic anions from plasma into the liver.
摘要
采用圆二色性方法检测大鼠肝细胞溶质中胆红素与配体蛋白的相互作用，以及在配体蛋白纯化过程中不同阶段获得的组分。在存在肝脏上清液成分的情况下，配体蛋白保留了结合胆红素的能力，但血清中结合胆红素的白蛋白在肝脏上清液中失去了结合胆红素的能力。这归因于配体蛋白表现出更高的结合特异性。在各自的生理环境中，白蛋白和配体蛋白在结构上适于结合配体：血清中的白蛋白和肝细胞溶质中的配体蛋白。这些研究与以下假设一致，即肝脏中配体蛋白的浓度可调节某些有机阴离子从血浆进入肝脏的净通量。
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