Fatranská M, Budai D, Gulya K, Kvetnanský R
Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Life Sci. 1989;45(2):143-9. doi: 10.1016/0024-3205(89)90288-9.
The aim was to study the mechanism of the previously established decrease in acetylcholine (ACh) concentration in the rat hippocampus under cold stress. Male rats were exposed for 14 days to cold (5 degrees C) or kept (controls) at room temperature (24 degrees C). Acetylcholine content, release and muscarinic receptor binding were investigated in the hippocampus. Cold exposure resulted in a decrease of ACh concentration in the dorsal hippocampus. Moreover, the potassium-evoked release of ACh from hippocampal slices was increased and an increase of maximal binding capacity of [3H] (-) quinuclidinyl benzilate in the dorsal hippocampus of cold exposed animals was also observed. Thus the decrease of hippocampal ACh concentration under cold exposure is probably due to its increased release. On balance then, our results demonstrate that cold stress in the rat induces significant activation of the hippocampal cholinergic system.
目的是研究先前确定的冷应激下大鼠海马中乙酰胆碱（ACh）浓度降低的机制。将雄性大鼠暴露于寒冷环境（5摄氏度）14天，或将其置于室温（24摄氏度）下作为对照。研究了海马中的乙酰胆碱含量、释放及毒蕈碱受体结合情况。冷暴露导致背侧海马中ACh浓度降低。此外，海马切片中钾离子诱发的ACh释放增加，并且在冷暴露动物的背侧海马中还观察到[3H]（-）喹核醇基苯甲酸酯的最大结合能力增加。因此，冷暴露下海马ACh浓度降低可能是由于其释放增加所致。总体而言，我们的结果表明，大鼠的冷应激会显著激活海马胆碱能系统。
