Toczyłowska-Mamińska Renata, Kloch Monika, Zawistowska-Deniziak Anna, Bala Agnieszka
Warsaw University of Life Sciences, Faculty of Wood Technology, Department of Physics, 159 Nowoursynowska St, Warsaw, Poland.
Warsaw University of Life Sciences, Faculty of Wood Technology, Department of Physics, 159 Nowoursynowska St, Warsaw, Poland.
Talanta. 2016 Oct 1;159:7-13. doi: 10.1016/j.talanta.2016.06.001. Epub 2016 Jun 2.
A novel construction of all-solid-state potentiometric sensor array designed for physiological measurements has been presented. The planar construction and elimination of liquid phase creates broad opportunities for the modifications in the sensing part of the sensor. The designed construction is based on all-solid-state ion-selective electrodes integrated with the ionic-liquid based reference electrode. Work parameters of the sensor arrays were characterized. It has been shown that presented sensor design indicates high sensitivity (55.2±1mV/dec, 56.3±2mV/dec, 58.4±1mV/dec and 53.5±1mV/pH for sodium-, potassium-, chloride- and pH-selective electrodes, respectively in 10(-5)-10(-1.5)M range of primary ions), low response time (t95 did not exceed 10s), high potential stability (potential drift in 28-h measurement was ca. ±2mV) and potential repeatability ca. ±1mV. The system was successfully applied to the simultaneous determination of K(+), Cl(-), Na(+) and pH in the model physiological solution and for the ion flux studies in human colon epithelium Caco-2 cell line as well.
本文介绍了一种专为生理测量设计的新型全固态电位传感器阵列结构。平面结构和液相的消除为传感器传感部分的改进创造了广阔的机会。所设计的结构基于与离子液体基参比电极集成的全固态离子选择性电极。对传感器阵列的工作参数进行了表征。结果表明，所提出的传感器设计具有高灵敏度（在10(-5)-10(-1.5)M的主要离子范围内，钠、钾、氯和pH选择性电极的灵敏度分别为55.2±1mV/dec、56.3±2mV/dec、58.4±1mV/dec和53.5±1mV/pH）、低响应时间（t95不超过10秒）、高电位稳定性（28小时测量中的电位漂移约为±2mV）和电位重复性约为±1mV。该系统已成功应用于模型生理溶液中K(+)、Cl(-)、Na(+)和pH的同时测定，以及人结肠上皮Caco-2细胞系中的离子通量研究。
