College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, PR China.
College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, PR China; Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou, Guangdong 510640, PR China.
Talanta. 2016 Oct 1;159:255-261. doi: 10.1016/j.talanta.2016.06.031. Epub 2016 Jun 16.
We develop a new lysosome-targeting AIE fluorescent probe tetraphenylethene-morpholine (TPE-MPL), by incorporating a typical lysosome-targeting moiety of morpholine into a stable tetraphenylethene skeleton. Due to both the AIE and antenna effects, TPE-MPL possesses superior photostability, appreciable tolerance to microenvironment change and high lysosome targeting ability. Our findings confirm that TPE-MPL is a well-suited imaging agent for targeting lysosome and tracking dynamic movement of lysosome. Moreover, due to its synthetic accessibility, TPE-MPL could be further modified as a dual-functional probe for lysosome, thereby gain further insight into the role of lysosome in biomedical applications.
我们开发了一种新型的溶酶体靶向 AIE 荧光探针四苯乙烯-吗啉（TPE-MPL），通过将典型的溶酶体靶向基团吗啉引入到稳定的四苯乙烯骨架中。由于具有 AIE 和天线效应，TPE-MPL 具有优异的光稳定性、对微环境变化的良好耐受性和高溶酶体靶向能力。我们的研究结果证实，TPE-MPL 是一种适合用于靶向溶酶体和跟踪溶酶体动态运动的成像剂。此外，由于其合成的可及性，TPE-MPL 可以进一步修饰为溶酶体的双功能探针，从而进一步深入了解溶酶体在生物医学中的作用。
