肺癌患者支气管肺泡灌洗液中的CD4+/CD25(高表达)/FoxP3+/CD127-调节性T细胞
CD4+/CD25(high)/FoxP3+/CD127- regulatory T cells in bronchoalveolar lavage fluid of lung cancer patients.
作者信息
Osińska Iwona, Stelmaszczyk-Emmel Anna, Polubiec-Kownacka Małgorzata, Dziedzic Dariusz, Domagała-Kulawik Joanna
机构信息
Department of Pathology, Medical University of Warsaw, Warsaw, Poland; Department of Pneumonology and Allergology, Medical University of Warsaw, Warsaw, Poland.
Department of Laboratory Diagnostics and Clinical Immunology of Developmental Age, Medical University of Warsaw, Warsaw, Poland.
出版信息
Hum Immunol. 2016 Oct;77(10):912-915. doi: 10.1016/j.humimm.2016.07.235. Epub 2016 Jul 27.
The aim of the study was to compare the presence of regulatory T cells (Tregs) in the local lung cancer environment versus systemic immune response based on the examination of bronchoalveolar lavage fluid (BALf) and peripheral blood (PB) from the same patient. 35 patients with lung cancer were investigated. Flow cytometry method with panel of antibodies: anti CD4/CD25/FoxP3/CD127 for Tregs identification was used. We observed significantly higher proportion of Tregs in the BALF than in PB (median 9.4 vs. 5.4%, p<0.05). The increased proportion of Tregs in patients with advanced disease and in adenocarcinoma was found. This study confirmed the usefulness of BALF analysis in evaluation of immune response in lung cancer. Detection of Tregs in the local tumour environment may have therapeutic relevance in individual indication for anti-cancer immune-therapies.
本研究的目的是基于对同一患者支气管肺泡灌洗液（BALf）和外周血（PB）的检测，比较局部肺癌环境中调节性T细胞（Tregs）的存在情况与全身免疫反应。对35例肺癌患者进行了研究。采用抗体组合的流式细胞术方法：使用抗CD4/CD25/FoxP3/CD127抗体鉴定Tregs。我们观察到BALF中Tregs的比例显著高于PB（中位数分别为9.4%和5.4%，p<0.05）。在晚期疾病患者和腺癌患者中发现Tregs比例增加。本研究证实了BALF分析在评估肺癌免疫反应中的有用性。在局部肿瘤环境中检测Tregs可能对个体抗癌免疫治疗指征具有治疗相关性。
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