Du Fangtao, Wang Xiaoning, Shang Bo, Fang Jifeng, Xi Yuting, Li Aijuan, Diao Yenze
Department of Traumatic Orthopedics, The Second People's Hospital of Liaocheng, TaiShan Medical College, Liaocheng 252600, China.
Department of Orthopedics, The People's Hospital of Gaotang, Liaocheng 252800, China.
Acta Biochim Pol. 2016;63(3):589-93. doi: 10.18388/abp.2016_1272. Epub 2016 Jul 30.
Spinal cord injury (SCI) is one of the most severe traumatic injuries that results in dysfunction of limbs and trunk below the damaged section. Recent studies have shown that gastrodin (GAS) could improve the recovery of SCI. In the current study, we aimed to examine the possible mechanism underlying the effect of GAS on recovery of SCI in rats. In rats with SCI, GAS improved locomotor functions and decreased permeability of blood-spinal cord barrier, as illustrated by increase of Basso-Beattie-Bresnahan scores and decrease of Evans blue leakage. In addition, GAS inhibited inflammation, as evidenced by decrease of proinflammatory cytokines, including tumor necrosis factor α (TNFα) and interleukin-1β (IL-1β) in rats following SCI. Moreover, increase of TBARS content and decrease of glutathione (GSH) content and superoxide dismutase (SOD) activities in SCI rats were inhibited by GAS. Furthermore, GAS enhanced mRNA expression of nuclear factor (erythroid-derived 2)-like 2 (Nrf2), catalytic subunit of γ-glutamylcysteine ligase (GCLc) and modified subunit of γ-glutamylcysteine ligase (GCLm). The data suggested that GAS may promote the recovery of SCI through the enhancement of Nrf2-GCLc/GCLm signaling pathway, and subsequent improvement of oxidative stress and inflammation, resulting in decrease of permeability of BSCB and improved recovery of locomotor function in rats with SCI. The results have provided novel insights into GAS-related therapy of SCI and associated neurodegenerative diseases.
脊髓损伤（SCI）是最严重的创伤性损伤之一，会导致受损节段以下的肢体和躯干功能障碍。最近的研究表明，天麻素（GAS）可以促进SCI的恢复。在本研究中，我们旨在探讨GAS对大鼠SCI恢复作用的潜在机制。在SCI大鼠中，GAS改善了运动功能，降低了血脊髓屏障的通透性，这表现为Basso-Beattie-Bresnahan评分增加和伊文思蓝渗漏减少。此外，GAS抑制了炎症，这可通过SCI大鼠中促炎细胞因子（包括肿瘤坏死因子α（TNFα）和白细胞介素-1β（IL-1β））的减少来证明。此外，GAS抑制了SCI大鼠中丙二醛（TBARS）含量的增加以及谷胱甘肽（GSH）含量和超氧化物歧化酶（SOD）活性的降低。此外，GAS增强了核因子（红系衍生2）样2（Nrf2）、γ-谷氨酰半胱氨酸连接酶催化亚基（GCLc）和γ-谷氨酰半胱氨酸连接酶修饰亚基（GCLm）的mRNA表达。数据表明，GAS可能通过增强Nrf2-GCLc/GCLm信号通路，随后改善氧化应激和炎症，从而降低血脊髓屏障的通透性并改善SCI大鼠的运动功能恢复。这些结果为SCI及相关神经退行性疾病的GAS相关治疗提供了新的见解。
